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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of fish for the test chemical :
1. and 2.The study was designed to assess the toxic effects of the test compound on the ZEBRA FISH(Danio rerio) .
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate
- Molecular formula: C29H36N2O2
- Molecular weight: 444.615 g/mole
- Substance type: Organic
- Physical state: maroon lustrous liquid
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
1)The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1 g/L. Hence, concentrations are 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,respectively.

2) Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
1.TEST ORGANISM
- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation (length definition, mean, range and SD):average 1.94 cm
- Weight at study initiation (mean and range, SD):0.1494 g (average)

ACCLIMATION
- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):During last 48 hours = 0

FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:Withdrawal of food 1 day before and during exposure

2.TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source:Koyal aquarium, Nagpur
- Age at study initiation (mean and range, SD): -
- Length at study initiation (length definition, mean, range and SD):average 1.79 cm
- Weight at study initiation (mean and range, SD):0.1204g (average)
- Method of breeding: -
- Feeding during test: -
- Food type:Standard brand feed,
- Frequency: once daily
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
1) and 2) 148.5 mg of CaCO3
Test temperature:
23 deg C
pH:
7.7
Salinity:
2) 6.41
Nominal and measured concentrations:
1) 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L
2) 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L
Details on test conditions:
1) and 2) TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- No. of organisms per vessel:8 fishes

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark


2)
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.312 - < 0.625 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.625 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate is likely to be toxic to fish atleast in the concentration range of 0.312 to 0.625 mg/l
Executive summary:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate .The studies are as mentioned below:

1) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >0.3125 mg/L but <0.625 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L

2) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material .The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.

Test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L. LC50 (96 hours) Experimental = 0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L.

Thus, based on the above summarised studies, Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range 0.312 to 0.625 mg/l

L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate can be classified as "Acute aquatic-1" for short term toxicity for fish

Description of key information

Short - term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate .The studies are as mentioned below:

1) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >0.3125 mg/L but <0.625 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L

2) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material .The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.

Test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L. LC50 (96 hours) Experimental = 0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L.

Thus, based on the above summarised studies, Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range 0.312 to 0.625 mg/l

L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate can be classified as "Acute aquatic-1" for short term toxicity for fish

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.625 mg/L

Additional information

Short - term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate .The studies are as mentioned below:

1) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >0.3125 mg/L but <0.625 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L

2) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material .The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.

Test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L. LC50 (96 hours) Experimental = 0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L.

Thus, based on the above summarised studies, Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range 0.312 to 0.625 mg/l

L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of Ethanaminium, N-​[4-​[[4-​(diethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​ethyl-​ & acetate can be classified as "Acute aquatic-1" for short term toxicity for fish

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