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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across: supporting information
Reason / purpose for cross-reference:
read-across: supporting information
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of fish for the test chemical :1. Short term toxicity test was performed for test material according t OECD guideline2.To evaluate short term toxicity of test material on fish
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material : Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate- Molecular formula : C20H24N3OC2H3O2- Molecular weight : 381.473 g/mol- Smiles notation : [N+]=1(c2ccccc2C(C)(C)C1\C=N\NCc1ccc(cc1)OC)C.C(C)(=O)[O-]- InChl : 1S/C20H23N3O.C2H4O2/c1-20(2)17-7-5-6-8-18(17)23(3)19(20)14-22-21-13-15-9-11-16(24-4)12-10-15;1-2(3)4/h5-12,14H,13H2,1-4H3;1H3,(H,3,4)- Substance type: Organic- Physical state: Liquid (Yellow)
Analytical monitoring:
not specified
Details on test solutions:
1.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. Hence, concentrations are 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name:ZEBRA FISH- Source:UNIQUE AQUARIUM, NAGPUR- Length at study initiation (length definition, mean, range and SD):average 1.94 cm- Weight at study initiation (mean and range, SD):0.1494 g (average)ACCLIMATION- Acclimation period:7 days- Type and amount of food during acclimation:Standard brand feed- Feeding frequency during acclimation:Daily once- Health during acclimation (any mortality observed):During last 48 hours = 0FEEDING DURING TEST- Food type:Standard brand feed- Frequency:Withdrawal of food 1 day before and during exposure
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
1) 148.5 mg of CaCO3
Test temperature:
1) 23 °C
pH:
1) 7.12
Dissolved oxygen:
1) 7.8 mg/l
Nominal and measured concentrations:
1) 0.3125mg/L, 0.625mg/L,1.25mg/L, 2.5mg/L, 5mg/L
Details on test conditions:
TEST ORGANISM- Common name:ZEBRA FISH- Source:UNIQUE AQUARIUM, NAGPUR- Length at study initiation (length definition, mean, range and SD):average 1.94 cm- Weight at study initiation (mean and range, SD):0.1494 g (average)ACCLIMATION- Acclimation period:7 days- Type and amount of food during acclimation:Standard brand feed- Feeding frequency during acclimation:Daily once- Health during acclimation (any mortality observed):During last 48 hours = 0FEEDING DURING TEST- Food type:Standard brand feed- Frequency:Withdrawal of food 1 day before and during exposure
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.312 - <= 0.625 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate is not likely to be toxic to fish in the concentration range of 0.3125 to 0.60 mg/l
Executive summary:

Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate.The studies are as mentioned below:

1.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical . The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. The lethal concentrations LC50 was determine to be >0.3125 mg/L but <0.625 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L.

2.Short term toxicity test for fish was performed to evaluate toxicity of test material on fish .The effect concentration of test material on fish was observed to be 0.60 mg/l after 96 h.

Thus, based on the above summarised studies, Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate and it’s functionally similar read across substance, it can be concluded that effect concetration of test substance is in the range of 0.3125 to 0.60 mg/l Thus, comparing this value with the criteria of CLP regulation, Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate can be classified as Aquatic acute/chronic 1 for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similar

read across, test chemical Ammonium iodide is not likely to be toxic atleast in the dose range of 405.0 mg/L .

 

Description of key information

Short term toxicity to fish:

Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate.The studies are as mentioned below:

1.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical . The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. The lethal concentrations LC50 was determine to be >0.3125 mg/L but <0.625 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L.

2.Short term toxicity test for fish was performed to evaluate toxicity of test material on fish .The effect concentration of test material on fish was observed to be 0.60 mg/l after 96 h.

Thus, based on the above summarised studies, Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate and it’s functionally similar read across substance, it can be concluded that effect concetration of test substance is in the range of 0.3125 to 0.60 mg/l Thus, comparing this value with the criteria of CLP regulation, Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate can be classified as Aquatic acute/chronic 1 for short term toxicity for fish .

 

Key value for chemical safety assessment

Additional information

Short term toxicity to fish:

Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate.The studies are as mentioned below:

1.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical . The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. The lethal concentrations LC50 was determine to be >0.3125 mg/L but <0.625 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L.

2.Short term toxicity test for fish was performed to evaluate toxicity of test material on fish .The effect concentration of test material on fish was observed to be 0.60 mg/l after 96 h.

Thus, based on the above summarised studies, Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate and it’s functionally similar read across substance, it can be concluded that effect concetration of test substance is in the range of 0.3125 to 0.60 mg/l Thus, comparing this value with the criteria of CLP regulation, Reaction mass of 3H-​Indolium, 2-​[[2-​(4-​methoxyphenyl)​-​2-​methylhydrazinyliden​e]​methyl]​-​1,​3,​3-​trimethyl-​ & acetate can be classified as Aquatic acute/chronic 1 for short term toxicity for fish .