Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride

Endpoint summary

Description of key information

Short term toxicity to fish:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on Zebra fish (Danio rerio) was determined to be 10 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic chronic 2 as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 54.8 mg/L for immobilisation effects.

2. The effect concentration EC50 was observed to be 22 mg/l for test chemical on daphnia magba after 24h of exposure.

Based on the both studies, it was concluded that the chemical reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic as the value ranges from 22-54-.8 mg/l and classified as aquatic chronic 3 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

1. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study.

2.The median effective concentration (ErC50) for the test substance, in a freshwater algae Desmodesmus subspicatus was determined to be 17.8 mg/L on the basis of effects on growth rate in a 72 hour study.

3.After 72 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be 98.169 mg/l graphically and through probit analysis.

Based on the above studies, it was concluded that the chemical reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Toxicity to microorganisms:

1. After the exposure of test chemical for 3 hrs, inhibitory concentration at which 50 % rate of respiration affects was measured. Based on the inhibition of respiration rate IC50 was observed in the range of 10-100 mg/l. Thus based on IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP criteria.

2. The IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be 10-100 mg/1 after 3 hrs of exposure.

Based on the above studies, it was concluded that the Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic.

Additional information

Summarized result for the toxicity of test chemical Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on the growth and other activity of fish, aquatic invertebrates and algae are as follows:

Short term toxicity to fish:

This study was designed to assess the toxic effects of the test reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with continuous one hr stirring, stock was analytically detected, solubility found 1015.78 mg/L. On the basis of solubility value following test concentrations prepared 1.25mg/L, 2.5mg/L, 5mg/L, 10mg/L, 20mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on Zebra fish (Danio rerio) was determined to be 10 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic chronic 2 as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of the test reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on the mobility of aquatic invertebrates. The studies are as mentioned below:

 

In first study from experimental study report determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The test substance was tested at the concentrations 0, 6, 10, 18, 32, 58 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, on Daphnia magna was determined to be 54.8 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.

 

First study was supported by the second from experimental source. Aim of this study was to evaluate the toxicity of test chemical on the biological activity and mobility of daphnia magna. Test conducted under the static system for 24 hrs. The effect concentration EC50 was observed to be 22 mg/l for chemical after 24h of exposure. Based on the EC50 value, chemical was consider as toxic and can be consider to be classified as aquatic chronic category 3 as per the CLP classification criteria.

 

Based on the both studies, it was concluded that the chemical reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of the test chemical reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride

on the growth of algae. The studies are as mentioned below:

 

In first study from experimental report 2016, freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to OECD Guideline 201. The stock solution (100 mg/L) was prepared by dissolving pinkish red powder in DMSO/ OECD growth medium. The stock solution was kept in ultrasonic bath for 20min Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 2.5, 5, 10, 20 and 40 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic 3 as per the CLP criteria.

 

 

Similarly in second study from the experimental report freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to OECD Guideline 201. The stock solution 2 g/L was prepared by dissolving yellow powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test higher substance concentrations due to solubility of the test substance and tested at the concentrations 0, 0, 0.5, 1, 2 and 4 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 17.8 mg/L. Based on the ErC50, it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

 

Above studies was supported by the third study. The effect of test item was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.2 5 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test solution was prepared in aseptic condition. The test item was prepared by adding 50 mg of test item in 250 ml of BBM to get the final concentration of 200 mg/L. This stock solution was kept for stirring for 20 minutes to obtain a homogenous solution for the experiment. The test concentrations were chosen according to the available data of the test item. The concentrations chosen were set up to the water solubility limit. The remaining test solutions were prepared by dilution from the above stock solution. To have a better growth and visibility of cells, the initial of the culture was kept 1 X 10000 cells/ml. Care was taken to have a homogeneous solution for the experiment. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 98.169 mg/L. This value indicates that the substance is toxic to algae and can be classified as Aquatic chronic category 3 as per the CLP criteria.

 

 

Based on the above studies, it was concluded that the chemical reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Based on the results, chemical Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was concluded as toxic and classified in chronic 3 as per the aquatic invertebrates and algae endpoint, but as the chemical was classified as chronic 2 in fish, thus on that basis chemical was consider as toxic and classified as 2 as per the CLP criteria.

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals study has been reviewed to determine the toxicity of the test chemical Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on the microorganisms. The studies are as mentioned below:

 

The first study was conducted to investigate the toxic nature of test chemical when comes in contact with microorganisms. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge only and with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. After the exposure of test chemical for 3 hrs, inhibitory concentration at which 50 % rate of respiration affects was measured. Based on the inhibition of respiration rate IC50 was observed in the range of 10-100 mg/l. IC50.

Similarly in second study, screening method based on the measurement of the respiration rate of activated sludge for assessing the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge and the respiration rate of activated sludge with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. Thus, IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be10 - 100 mg/1 after 3 hrs of exposure.

 

Based on the above studies, it was concluded that the Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic.