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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetat on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic.

Short-term toxicity to aquatic invertebrate:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

2. The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be 0.116 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

Thus, based on the above summarised studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concetration value is in the range of 0.03 - 0.116 mg/ L. Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be classified for as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria:

1. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 0.13 mg/L on the basis of effects on growth rate in a 72 hour study.

2. After 72 hours of exposure to test item to various nominal test concentrations, EC50 was found to be 0.610mg/l graphically and through probit analysis.

Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be classified as toxic for short term for aquatic algae and cyanobacteria as and classified aq aquatic acute 1 as per the CLP criteria.

Toxicity to microorganisms:

1. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.

2. The effect of test material on the survival time of Paramaecium caudatum was measured microscopically for 20 min. Concentration of test material in the bathing fluid was 0.1 or 1 .0%.

During the experiment the 36.7% mortality observed of test organism i.e Paramecium caudatum after of 17.28 min.which is not consider to be very toxic.

Thus based on the above studies chemical was consider toxic.

Additional information

Short term toxicity to fish:

This study was designed to assess the toxic effects of the test compound Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with continuous 1 hr stirring. After stirring stock was analytically detected and the solubility was found 672.34 mg/L, on the basis of this value following test concentration was prepared 0.1562mg/L, 0.3125mg/L, 0.625mg/L, 1.25mg/L,2.5mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic acute 1 chronic 1 as per the CLP criteria.

Short-term toxicity to aquatic invertebrate:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate. The studies are as mentioned below:

 

In the key study from the experimental report 2016 toxicity was determine. Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution 20 mg/L was prepared by dissolving dark violet liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 as per the CLP criteria.

 

Similarly the first study was supported by the second. Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution (10 mg/L) was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.016 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic acute category 1 as per the CLP criteria.

 

Thus, based on the above summarized studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concentration value is in the range of 0.03 - 0.116 mg/ L. Thus, comparing this value with the criteria of CLP regulation Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be classified for as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

 

 

 

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate.The studies are as mentioned below:

 

In the key study from the experimental report 2016 freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201.The stock solution (10 mg/L) was prepared by dissolving yellow powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Determination of cell concentrations was performed by microscope with counting chamber Cyrus I or electronic particle counter. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, test substance, in Desmodesmus subspicatus was determined to be 0.13 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic acute category 1 as per the CLP criteria.

 

The first study was supported by the second experimental study for read across chemical. The effect of test item was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 0.5mg/l, 1.25mg/l, 3.125mg/l, 7.813mg/l, 19.531mg/l and 48.828mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 0.610 mg/L. Thus, based on the EC50 value, test substance can be considered as toxic to aquatic organisms and thus can be classified as aquatic acute category 1 as per the CLP criteria.

 

Thus, based on the above summarized studies, Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate it can be concluded that effect concentration value is in the range of 0.13 - 0.61 mg/L. Thus, comparing this value with the criteria of CLP regulation reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate can be classified as toxic for short term for aquatic algae and cyanobacteria as and classified aquatic acute 1 as per the CLP criteria.

Toxicity to microorganisms:

Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity microorganisms of the test Reaction mass of Methylium, tris[4-​(diethylamino)​phenyl]​-​ & acetate.The studies are as mentioned below:

Determination of toxicity of test material on the growth of 14 gram negative microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM). 14 different gram negative bact. were used for the study was Agrobacterium radiobacter, Agrobacterium tumefaciens, Bradyrhizobium japonicum, Escherichia coli, Erwinia atroseptica,Erwinia herbicola, Erwinia uredovora, Pseudomonas fluorescence, Pseudomonas phaseolicola, Pseudomonas syringae , Rhizobium trifolii, Xanthomonas malvacearum, Xanthomonas phaseoli and X. stewartii. These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.

The effect of test material on leucine aminopeptidase, acid phosphatase, and y-glutamyl transpeptidase activity in P. caudatum was studied in order to investigate the mechanism of toxicity.

The effect of test material on the survival time of Paramaecium caudatum was measured microscopically for 20 min. Concentration of test material in the bathing fluid was 0.1 or 1 .0%.

During the experiment, 36.7% mortality observed of test organism i.e Paramecium caudatum after exposure of 17.28 min. Thus on that basis chemical was not consider to be very toxic.

Thus based on the above studies chemical was consider toxic.