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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

In a key study the ready biodegradability of the test substance was determined according to the EEC-Directive 92/69 (1992). The biodegradation degree (DOC removal) after 28 days was determined to be 80 – 90 %. The test substance is in this test readily biodegradable according to OECD criteria.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

In a key study the ready biodegradability of the test substance was determined according to the EEC-Directive 92/69 (1992); equivalent to OECD Guideline 301 A (1993) and ISO Standard 7827 (1994). The biodegradation of the test substance was determined by exposure to activated sludge from laboratory wastewater plants treating municipal and synthetic sewage. The concentration of the test substance was 124 mg/L (equivalent to 20 mg/L DOC) and the concentration of the inoculum was 30 mg/L (dry weight). As a reference compound (procedure control), aniline was tested simultaneously under the same conditions. In the toxicity control, containing both test substance and the reference compound aniline, no inhibitory effect on the microorganisms was observed. In the procedure controls, the reference compound aniline was readily biodegraded by an average of 90-100 % after 14 of exposure. All validity criteria were fulfilled. At the end of the test, the test substance was biodegraded by an average of 80-90 %. Therefore, the test substance was considered to be readily biodegradable according to OECD criteria.