3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Apr - 27 Jun 2007

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Version / remarks:

Adopted 14. Jun 1996

Deviations:

yes

Remarks:

No depuration phase

Qualifier:

according to guideline

Guideline:

other: “Method for testing the degree of accumulation of chemical substances in fish body“ stipulated in the “Testing for new chemical substances” (November 21, 2003, No. 1121002, Ministry of the Environment, Japan)

GLP compliance:

yes

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: Fish were analysed five times during test duration.
- Sampling intervals/frequency for test medium samples: Test water was analysed once before first analysis of test fish and thereafter.
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods): Control fish were analyzed before the experimental start and after the experimental completion. Four fish were taken at each sampling time and divided into two groups and analysed individually. In addition, six fish were taken out for measurement of lipid content.

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Level 1: 1000 mg/L stock solution was prepared by dissolving the test substance and Tween-80 (10 times amount of it) in N,N-dimethylformamide. 40 mg/L solution of the test item was then prepared from this stock solution by dilution with N,N-dimethylformamide; Level 2: 4 mg/L stock solution of the test item was prepared from stock solution of Level 1 by dilution with N,N-dimethylformamide.
- Controls: Tween 80 was dissolved in N,N-dimethylformamide to prepare a 400 mg/L stock solution as Tween 80 concentration
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80; N,N-dimethylformamide

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Source: Karume laboratory chemicals evluation and research institute, Fukuoka, Japan (date of hatch: 3 Sep 2006)
- Age at study initiation (mean and range, SD): yearling fish
- Length at study initiation (length definition, mean, range and SD): 6.8 - 10.9 cm
- Lipid content at test initiation (mean and range, SD): 3.64%
- Health status: Only healthy fish were used for testing.
- Description of housing/holding area: After rearing to test fish size in an aquarium, the fish were transferred to an acclimatizing aquarium and acclimatized after the external disinfection. The fish showing any abnormality were removed and the remainder was reared for 56 d in a flow-through system at 25 +/- 2 °C. Fish were checked for health conditions and transferred to test tanks. Thereafter, the fish were reared at the same temperature in a flow through system for 14 d following external disinfection. The external disinfeciton in the acclimatizing aquarium was carried out in an aqueous solution containing 20 mg/L ELBAZIU and 7 g/L sodium chloride for 24 h. The external disinfection in the test tanks was carried out in an aqueous solution containing 20 mg/L ELBAZIU and 7 g/L sodium chloride for 24 h
- Feeding during test
- Food type: Feed for fry of carp (proteins: ≥ 43%, lipid content: ≥ 3%, manufacturer: Nippon Formula Feed Mfg. C., Ltd., Japan)
- Amount: 2% of total body weight
- Frequency: Twice a day in halves (once a day in all at holiday)

ACCLIMATION
- Acclimation period: 70 d
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Feed for fry of carp (proteins: ≥ 43%, lipid content: ≥ 3%, manufacturer: Nippon Formula Feed Mfg. C., Ltd., Japan)
- Feeding frequency: Twice a day in halved (once a day in all at holiday)
- Health during acclimation (any mortality observed): no; unhealty fish were removed

Route of exposure:

aqueous

Justification for method:

aqueous exposure method used for following reason: The substance has a low potential for adsorption (log Koc 2.65) and a water solubility of 1.21 mg/L. Thus, the aqueos exposure route is considered relevant.

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

60 d

Total depuration duration:

0 d

Hardness:

14.2 mg/L (Ca, Mg)

Test temperature:

Level 1: 24.2 - 24.8 °C
Level 2: 24.0 - 24.6 °C
Control: 24.2 - 24.6 °C

pH:

Level 1: 7.7
Level 2: 7.7 - 8.0
Control: 7.9 - 9.1

Dissolved oxygen:

Level 1: 6.9 - 7.3 mg/L
Level 2: 6.9 - 7.3 mg/L
Control: 7.4 - 8.0 mg/L

TOC:

0.3 mg/L

Conductivity:

278 µS/cm

Details on test conditions:

TEST SYSTEM
- Test vessel: 70 L glass tanks for volatile substances
- Renewal rate of test solution (frequency/flow rate): 0.05 mL/min for stock solution and 2000 mL/min for dilution water, 2880 L/day if test water were supplied; Stock solution: 1 L glass bottle cooled in a refrigerator (frequency of renewal: 1-2 times/week)
- No. of organisms per vessel: 47 for Level 1 + 2, 18 for control
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Groundwater from the premises of the laboratory
- Metals: < standard value
- Pesticides: not detected
- Chlorine: not detected
- Alkalinity: 101 mg/L
- Holding medium different from test medium: no
- Intervals of test medium replacement: flow-through

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h light / 10 h dark
- Light intensity: artificial light of white fluorescent lamp

Nominal and measured concentrations:

Nominal: 1 µg/L (Level 1), 0.1 µg/L (Level 2)
Measured: 0.929 µg/L (average; SD: 0.0482 µg/L, Level 1), 0.0913 µg/L (average; 0.00611 µg/L, Level 2)

Reference substance (positive control):

no

Lipid content:

3.64 %

Time point:

start of exposure

Remarks on result:

other: Lipid content was determined with gravimetric analysis after chloroform-methanol extraction (n=6).

Lipid content:

4.83 %

Time point:

end of exposure

Remarks on result:

other: Lipid content was determined with gravimetric analysis after chloroform-methanol extraction (n=6).

Conc. / dose:

1 µg/L

Temp.:

>= 24.2 - <= 24.8 °C

pH:

7.7

Type:

BCF

Value:

380 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Conc. / dose:

0.1 µg/L

Temp.:

>= 24 - <= 24.6 °C

pH:

7.85

Type:

BCF

Value:

300 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

not measured/tested

Remarks:

No depuration time was included in the study.

Remarks on result:

not measured/tested

Remarks:

No rate constants were calculated.

Metabolites:

Since the substance is expected to be stable based on its chemical structure no analysis of possible metabolites was performed.

Details on results:

- Behavioural abnormalities: No abnormalities were observed.

Table 1: Measured concentrations of test item in water [µg/L]

Level	After 4 d	After 11 d	After 24 d	After 38 d	After 47 d	After 60 d	Average (SD)
1	0.978	0.879	0.915	0.993	0.927	0.879	0.929 (0.0482)
2	0.0954	0.0979	0.0870	0.0696	0.0835	0.0873	0.0913 (0.00611)

 

Table 2: Variation of BCFs

Level	-	After 38 d	After 47 d	After 60 d	Average of three results
1	Average BCF	354.94	421.30	347.42	374.55
	Variation rate from the average of three results (%)	5.2366	12.48	72440	-
2	Average BCF	329.45	285.93	282.00	299.13
	Variation rate from the average of three results (%)	10.137	4.4107	5.7270	-

 Since the variation of BCF values were within ± 20% of the average, it was concluded that a steady state was reached. The BCFss was calculated on the basis of the results presented in Table 2.

Table 3: Measured concentrations of test item in water at the steady state [µg/L]

Level	After 4 d	After 11 d	After 24 d	Average
1	0.993	0.927	0.879	0.933
2	0.0969	0.0835	0.0873	0.0892

Table 4: Validity criteria

Criterion according to guideline	Outcome	Fulfilled?
The temperature variation is less than ± 2 °C	Level 1: 24.2 - 24.8 °C Level 2: 24.0 - 24.6 °C Control: 24.2 - 24.6 °C	Yes
The concentration of dissolved oxygen does not fall below 60% saturation.	At least > 80% based on the reported concentrations of dissolved oxygen in test water.	Yes
The concentration of the test substance in the chambers is maintained within ± 20% of the mean of the measured values during the uptake phase.	83.5 - 99.3%	Yes
The mortality or other adverse effects/disease in both control and treated fish is less than 10% at the end of the test; where the test is extended over several weeks or months, death or other adverse effects in both sets of fish should be less than 5% per month and not exceed 30% in all.	No mortality was reported.	Yes

Validity criteria fulfilled:

yes

Remarks:

Please refer to Table 4 ("Any other information on results incl. tables") for detailed information.

Description of key information

BCF: 300 - 380 based on whole body wet weight

Additional information

One experimental study is available investigating the bioaccumulation of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene (CAS 25291-17-2) in fish (Daikin Industries, 2007). The study was performed according to OECD 305 (1996) and GLP with Cyprinus carpio (yearling fish) as test organism. Fish were exposed via the aqueous phase to 0.1 and 1.0 µg/L for a duration of 60 d without a depuration phase. Aqueous exposure was considered to be the relevant exposure route since adsorption of the substance to particulate matter is expected to be low. Due to volatility of the test item special test vessels were used to avoid volatilization. Moreover, a flow-through test design with a high daily renewal rate of 2880 L/d was used in order to maintain test concentrations constant during exposure. The actual concentrations were measured several times during exposure and resulted in concentrations close to nominal (average: 0.929 µg/L, Level 1; 0.0913 µg/L, Level 2). For preparation of test solutions the solubiliser Tween-80 (10 times) in N,N-dimethylformamide was used. The concentration of the solubilizing agent was below 0.1 mg/L as recommended in the guideline.
The steady-state was reached within the duration of the study since measurements after 38, 47 and 60 d showed that the variation of BCF values were within ± 20% of the average. The calculated BCF-values were 380 (Level 1, 1 µg/L) and 300 (Level 2, 0.1 µg/L) based on whole body wet weight. This result indicates a low potential for bioaccumulation via the aqueous phase.