1,4-dichloro-5,8-dihydroxyanthraquinone

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Principles of method if other than guideline:

No specific Guideline stated.

GLP compliance:

no

Remarks:

Study conducted prior to implementation of GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichloro-5,8-dihydroxyanthraquinone

EC Number:

220-599-4

EC Name:

1,4-dichloro-5,8-dihydroxyanthraquinone

Cas Number:

2832-30-6

Molecular formula:

C14H6Cl2O4

IUPAC Name:

1,4-dichloro-5,8-dihydroxy-9,10-dihydroanthracene-9,10-dione

Test material form:

solid

Test animals

Species:

rat

Strain:

other: Wistar TNO W 74

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 9-14 weeks
- Weight at study initiation: 175 g (mean weight male/female)
- Housing: makrolon cages (type III)
- Diet (e.g. ad libitum): Altromin R 1324
- Water (e.g. ad libitum): tap water

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5
- Humidity (%): 60 +/-5
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

poloxamer

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw

Doses:

one single dose of 5 g/kg bw

No. of animals per sex per dose:

5 male, 5 female

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: multiple on day of application, followed by twice a day (once at weekends and bank holidays); weighing on day of application and at end of observation period
- Necropsy of survivors performed: no information
- Other examinations performed: observation of clinical signs during the observation period

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

none

Body weight:

no impact

Gross pathology:

no information

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The oral LD50 of 5,8-Dichlorchinizarin in male and female rats was > 5000 mg/kg bw.

Executive summary:

In an acute oral toxicity study, 5 female and 5 male, 9-14 weeks old Wistar strain rats were given a single oral dose of 5,8-Dichlorchinizarin in poloxamer (Lutrol) by gavage at a dose of 5000 mg/kg bw and observed for 14 days.

During the observation period, no death, no clinical signs and no impact on weight were observed in any animal.

Oral LD50 (rat, male/female) > 5000 mg/kg bw