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EC number: 223-026-6 | CAS number: 3697-42-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- cited as 92/69/EEC (C.2.) (1992)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Infracor Chemistry Services
- Analytical monitoring:
- yes
- Details on sampling:
- The analyses were carried out by measurement of dissolved organic carbon.
The test item concentration range for the testing was below the detection limit of 1 mg TOC/L. Beside this, no suitable substance specific method exist at this time to analyse the test item concentrations in this very low range.
Due to these facts, only the test item concentrations of the stock solution and one of the additional test concentration directly above the detection limit (= additional stability control) could be determined after 0 and 48 h by TOC analysis. These measurements were used to assess the stability of the test item in this test system. The results of the stock solution analysis were used for the calculation of the nominal amount of the test item in the test vials. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Strain: clone 5
- Source: Animals bred at the testing facility, originally derived from Dr. N. Caspers, Bayer AG, Leverkusen, Germany
- Age at study initiation: Neonates < 24 h old
- Method of breeding: For breeding the females were maintained in M4-medium in 1L beakers.
At two to three day intervals, the exuviae were syphoned off, the water was changed and the offspring
removed from the breeding vessels.
At regular intervals of approximately 4 weeks, offspring was isolated from the vessels. They served as
a basis for further breeding.
Females are separated from the offspring 24 hours before the start of the test. The litter produced
overnight was used for the test.
The culture conditions ensure parthenogenetic reproduction.
- Feeding during test: no
ACCLIMATION
- Food type: Desmodesmus (formerly Scenedesmus) subspicatus, unicellular green algae
- Amount: Amount of food was such that the amount added was consumed by the animals
- Frequency: Daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 14°dH = 250 mg CaCO3/L
- Test temperature:
- 20 +/- 1°C
- pH:
- 7.8 - 8.1
- Dissolved oxygen:
- 7.7 - 8.2 mg/L
- Nominal and measured concentrations:
- 0.0077, 0.013, 0.023, 0.040, 0.070, 0.12 mg/L (concentration are based on the main product)
The analytical concentrations of the stock solution and the additional stability control after 48 h did not deviate by more than 20% from the freshly prepared solutions. It can be concluded that the test substance concentration was stable during the whole exposure time. Therefore, the nominal concentration of the stock solution was used to calculate the test item concentrations in the test batches. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 mL
- Type: open. Test substance is not volatile
- No. of organisms per vessel: 5 +/- 1
- No. of vessels per concentration (replicates): 4
- Biomass loading rate: Approx. 2 mL per test organisms
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water
- Ca/Mg ratio: 4 : 1
- Culture medium different from test medium: Yes, Elendt M4 medium as described in OECD Guideline 202
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Complete darkness
EFFECT PARAMETERS MEASURED : Immobility. Sampling at 24 and 48 hours - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.04 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.055 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: range of EC50: 0.04 - 0.07 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.12 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
EC50 (24 h) > 1 mg/L (nominal concentrations)
EC100 (24 h) = 2 mg/L - Reported statistics and error estimates:
- EC50 -values were determined graphically
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test results indicate that Chlorhexidine dihydrochloride is acutely very toxic towards daphnia. The 48h-EC50 was determined to be 0.055 mg a.s./L.
Reference
Description of key information
An GLP acute toxicty study with Daphnia magna according to the OECD 202 guideline was performed.
The test results indicate that Chlorhexidine dihydrochloride is acutely very toxic towards daphnia. The 48h-EC50 was determined to be 0.055 mg a.s./L.Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.055 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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