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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989-05-04 to 1989-05-11
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guidelineopen allclose all
Guideline:
other: Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms
Version / remarks:
USEPA, 1985 (EPA/600/4-85/014)
Guideline:
other: North Carolina Ceriodaphnia Chronic Effluent Bioassay Procedure
Version / remarks:
December 1985, revised June 1988, North Carolina Department of Natural Resources and Community Development, Division of Environmental Management, Water Quality Section
Principles of method if other than guideline:
- Principle of test: the test item is diluted 5:1 with spring water and treated with sludge from City of Columbis WWTP in 1:4 ratio. Solution is aerated for 24 hours and filtered before tested. In an additional test the sludge without any test item is tested for toxicity.
- Parameters analysed / observed: mortality, offspring
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dye Preparation: 30 mg/L dye diluted 5:1 with spring water and treated with sludge from City of Columbis WWTP in 1:4 ratio. Solution aerated for 24 hours and the solution filtered before tested.
- Controls: one control 100% spring water, additional test for control of toxicity of sludge
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): stock solution was filtrated before using for dilutions

Test organisms

Test organisms (species):
Ceriodaphnia sp.
Details on test organisms:
TEST ORGANISM
- Age at test start: 2 to 24 hours
- Feeding during test: yes
- Food type: green alga Selenastrum capricornutum
- Amount: at rate of approx. 2000000 cells/mL per day

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d
Remarks on exposure duration:
test solutions were renewed on days 2 and 5 with reproduction counts made at that time.

Test conditions

Test temperature:
25 +/- 1°C
pH:
6.92 - 7.60
Dissolved oxygen:
6.50 - 8.50 ppm
Nominal and measured concentrations:
control, and dilutions of 5, 15, 30 and 50% of the stock solution
Details on test conditions:
TEST SYSTEM
- Test vessel: 1ounce filled with 15 mL of solution
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

OTHER TEST CONDITIONS
- Photoperiod: 16h light/ 8h darkness
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
50 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: corresponding to 2.25 mg/L
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
50 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Sludge control: the test was performed in the same way as the test with test item.
Concentrations of sludge (without test item): 5, 15, 30, 50% and a control.
Result: sludge is not chronically toxic to Ceriodaphnia at the tested concentrations.

Any other information on results incl. tables

Results of the main test

Concentration of the test item Average Offspring Mortality
[%]
Control 15 10
5% 19 0
15% 20 0
30% 17 10
50% 15 10

Results of the sludge control test

Concentration of sludge Average Offspring Mortality
[%]
Control 15 10
5% 16 0
15% 20 0
30% 19 0
50% 26 0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Mortality in negative control 10%, average # offspring = 19
Conclusions:
The 7-d NOEC (reproduction) to Ceriodaphnia sp. was determined at 2.25 mg/L nominal.