Sulfuric acid, mono-C12-14-alkyl esters, magnesium salts

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

12 Nov - 15 Nov 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Stamm Berlin 33/2 (DSM 50026)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Post exposure observation period:

not relevant

Test temperature:

21 °C

pH:

6.6 - 6.8

Nominal and measured concentrations:

nominal: 0, 1000, 3000 and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Clim-o-shake at 100 rpm

EFFECT PARAMETERS MEASURED: growth inhibition, measured photometrically at 436 nm (Zeiss filterphotometer PL4)

Reference substance (positive control):

no

Duration:

16 h

Dose descriptor:

EC0

Effect conc.:

409 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

1 083.85 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

0.58% inhibition at 1000 mg prod./L

15.3% inhibition at 3000 mg prod./L

60.63% inhibition at 10000 mg prod./L

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

respiration test according to OECD 1976

Deviations:

no

Principles of method if other than guideline:

The respiration inhibition of the test substance to a sludge concentration of 1.5 g/L suspended solids is recorded after 3 hours.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge

Details on inoculum:

- Initial biomass concentration: 1.5 g/L suspended solids
- Source: Rungsted municipal treatment plant, Denmark, treating almost exclusively domestic sewage

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Reference substance (positive control):

not specified

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

135 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

188 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Refer to the Category Approach Justification document provided in IUCLID6 Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

135 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

Source, key, 151-21-3, 1983

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

188 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

Source, key, 151-21-3, 1983

Description of key information

3h EC50 (activated sludge, domestic) = 135 mg/L (respiration inhibition test, similar to OECD 209)

Key value for chemical safety assessment

EC50 for microorganisms:

135 mg/L

Additional information

The read-across to the structurally related category member was conducted in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5. The key, non-GLP test with C12AS Na (CAS 151-21-3) was performed according to a guideline similar to OECD 209. The EC50 values of 188 and 135 mg/L (nominal concentrations) were obtained for the endpoint respiration inhibition after 30 minutes and 3 hours of exposure, respectively. The 3-hour EC50 value of 135 mg/L, being the most sensitive value, was used for the PNEC STP derivation of C12-14AS Mg (CAS 90583-23-6).

Moreover, a P. putida test is available for C12-14AS TEA (CAS 90583-18-9; BASF 1992). In this study, the growth inhibition of Pseudomonas putida was recorded after approximately 16 hours of exposure. The 16-h EC10 value was determined to be 1084 mg a.i./L (nominal concentration).