Butyl glycollate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June12, 2019 - October 14, 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material: 120001168816
- Expiration date of the batch: May 12, 2020
- Purity test date: May 13, 2019

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Tightly closed in cool, dry, well ventilated area, refrigerator at 2-10°C

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Samples were taken from all test concentrations
- Sample storage conditions before analysis: Deep frozen (<= -18°C)

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: Stock culture
- Length at study initiation: 1.22-1.99 cm, mean: 1.64 cm
- Weight at study initiation: 0.0232-0.0838 g/fish, mean: 0.0520 g/fish

FEEDING DURING TEST
- Fish were not fed during the test period

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

164.3 mg/ CaCO3

Test temperature:

23.2 - 23.4°C

pH:

7.4 - 7.6

Dissolved oxygen:

94 - 107% of air saturation

Conductivity:

808 µS/cm²

Nominal and measured concentrations:

Nominal: 3.13, 6.25, 12.5, 25.0 and 50.0 mg/L
Measured: 2.59, 5.76, 11.4, 21.4 and 45.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 3.8L glass vessels
- Type: open
- Material, size, headspace, fill volume: Glass (covered with glass lids), 2.0 L fill volume, depth about 9 cm
- Aeration: Permanent
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.182 g/L

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Local tap water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 14 h light / 10 h dark
- Light intensity: 100-1000 lx; range: 455-575 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Effects on appearance, size and behaviour were observed daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0
- Range finding study : yes
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

23.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

29.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Biological observations: Lying on the bottom of the vessel, loss of equilibrium, swimming upside down, reduced swimming activity compared to the control, changes in ventilation rate and respiration
- Mortality of control: 0

Reported statistics and error estimates:

Weibull & Probit analysis was used for the determination of the LC50.
The NOEC and the LOEC were determined Step-down Cochran-Armitage Test Procedure.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the test, the LC50 was determined to be 23.1 mg test item /L. As the test concentrations did not remain within the range of +/- 20% of the nominal concentration, the LC50 value is based on the geometric mean measured values.

Description of key information

LC50 (zebrafish), based on mortality: 23.1 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

23.1 mg/L

Additional information

A 96-hour acute toxicity study in Zebrafish (Danio rerio) conducted under static conditions reported an LC₅₀ of 23.1 mg/l (geometrical mean measured concentrations) based on mortality.