Reaction mass of N-[3-(dimethylamino)propyl]stearamide and N-[3-(dimethylamino)propyl]hexadecan-1-amide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

August 13 - September 3 1990

Reliability:

4 (not assignable)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

A new version of the guidance has been published since the release of the study report.

GLP compliance:

not specified

Specific details on test material used for the study:

Test material: DMAPSA (Octadecanamide, N-(3-(dimethylamino)propyl)-) (CAS No. 7651-02-7).
Date of receipt: 24 July 1990.
Description: White waxy solid.
Storage: In original container at room temperature.

Vehicle:

yes

Details on test solutions:

Method of preparation: Preliminary solution in 10% Tween 80-ethanol.
Media renewal: Daily.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Test species: Rainbow trout (Salmo gairdneri).
Length: 4.8 ± 0.2 cm.
Weight: 1.56 ± 0.2 g. Loading: 0.78 g bodyweight/litre.
Supplier: Parkwood Trout Farm, Kent, U.K.
Mortalities: Zero.
Medication: None.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Approximately 50 mg/L as CaCO3.

Test temperature:

14 ± 1°C.

Nominal and measured concentrations:

RANGE FINDING STUDY:
Nominal concentrations: 0.1, 1, 10 mg/L.

DEFINITIVE STUDY:
Nominal concentration: 0.1 mg/L (two repetitions).

Details on test conditions:

Test water: Dechlorinated tap water (sodium thiosulfate).
Test vessels: 20L.
Aeration: Yes.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Please refer to the tables in "Any other information on results incl. tables".

Sublethal observations / clinical signs:

Table 1: Range-finding study

Concentration (mg/L)	Cumulative mortality (initial population = 2)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
0.1	0	0	0	0
1	2	2	2	2
10	2	2	2	2

Table 2: Definitive study

Concentration (mg/L)	Cumulative mortality (intial population = 10)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
0.1, repetition 1	0	0	0	0
0.1, repetition 2	0	0	0	0

Validity criteria fulfilled:

not specified

Conclusions:

The 96-hour LC50 value for acute toxicity to fish of DMAPSA (Octadecanamide, N-(3-(dimethylamino)propyl)-) was determined to be between 0.1 and 1 mg/L.

Executive summary:

The acute toxicity to fish (Oncorhynchus mykiss) of DMAPSA (Octadecanamide, N-(3-(dimethylamino)propyl)-) was evaluated.

A preliminary test on series of two fishes indicated that no mortality was expected for a test item concentration of 0.1 mg/L after a period of 4 days. Mortality was however observed for the two individuals at test item concentrations of 1 and 10 mg/L.

A definitive study was carried out on two series of 10 individuals with a concentration of 0.1 mg/L of test item for a period of 4 days. Two repetitions were performed. No mortality was observed.

Based on these results, it was concluded that the 96-hour LC50 value for acute toxicity to fish of DMAPSA (Octadecanamide, N-(3-(dimethylamino)propyl)-) is between 0.1 and 1 mg/L.

Description of key information

The acute toxicity to fish (Oncorhynchus mykiss) of DMAPSA (Octadecanamide, N-(3-(dimethylamino)propyl)-) was evaluated.

A preliminary test on series of two fishes indicated that no mortality was expected for a test item concentration of 0.1 mg/L after a period of 4 days. Mortality was however observed for the two individuals at test item concentrations of 1 and 10 mg/L.

A definitive study was carried out on two series of 10 individuals with a concentration of 0.1 mg/L of test item for a period of 4 days. Two repetitions were performed. No mortality was observed.

Based on these results, it was concluded that the 96-hour LC50 value for acute toxicity to fish of DMAPSA (Octadecanamide, N-(3-(dimethylamino)propyl)-) is between 0.1 and 1 mg/L. According to the Guidance on the Application of the CLP criteria (Guidance to Regulation (EC) No 1272/2008 on classification, labelling and packaging (CLP) of substances and mixtures, Version 5.0, July 2017), DMAPSA should therefore be classified as Very Toxic to Aquatic Life (H400, Aquatic acute Category 1) and Very Toxic to Aquatic life with long lasting effects (H410, Aquatic chronic Category 1) based on the CLP regulation criteria, with an M-factor of 1.