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EC number: 303-757-8 | CAS number: 94213-53-3
A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.
The reference EC50 value to set the limit test concentration was obtained from the Acute Toxicity to Daphnia magna study; the test was conducted at a single concentration of 98 mg/l.
Seven fish were exposed to an aqueous solution of the test item, at a single concentration of 98 mg/l for a period of 96 hours at a temperature of 14 to 15 ºC under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 1, 3 and 6 hours after the start of exposure, and then daily throughout the test until termination after 96 hours.
Chemical analysis of the fresh test preparations at 0 and 72 hours, and the aged test preparations at 24 and 96 hours, showed measured test concentrations to range from 98 to 99 % of nominal, and so the results are based on nominal test concentrations only.
Exposure of rainbow trout to the test item gave LC50 values of greater than 98 mg/l. The No Observed Effect Concentration was 98 mg/.
LC50 (96h) > 98 mg/l (nominal)
Not harmful/toxic for short-term exposure to fish
The substance (commercial formulation) was assayed for the short-term toxicity potential to fish, accordign to the ETAD reccomendations. Unfortunately, only a sheet of test results is currently available; therefore, there are no details about actual test conditions and results. The information has been here mentioned for completeness sake.
The Lethal Concentration for 0 % of population was identified to be 456 mg/l, while the 100 % of mortality was indicated to be caused by 870 mg/l. The CL50 was reported to be 663 mg/l (sheet of results, 1987).
In order to clarify the short-term toxicity to fish potential of Direct Violet 051, the available data on structural analogous Similar Substance 01 have been taken into account. The read across approach can be considered as reliable and adequate for the purpose; details and explanations are detailed in the report attached to the IUCLID section 13.
A study was performed with rainbow trout, according to the OECD Guideline No 203. The reference EC50 value to set the limit test concentration was obtained from the Acute Toxicity to Daphnia magna study; the test was conducted at a single concentration of 98 mg/l. No deaths were recorded overt the 96 hours exposure period. Therefore, the No Observed Effect Concentration (NOEC) was 98 mg/l. A sub-lethal effect of exposure was observed in the 98 mg/l test concentration: the fish were observed to have a slight staining on the fins and around the mouth, but otherwise were observed to be normal.
At the start and throughout the test the control was observed to be a clear, colorless solution; however, the 98 mg/l test solution was observed to be a dark, purple solution.
Chemical analysis of the fresh test preparations at 0 and 72 hours and the aged test preparations at 24 and 96 hours, showed measured test concentrations to range from 98 to 99 % of nominal, and so the results are based on nominal test concentrations only.
Complete reference are included into the CSR file.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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