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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Read across to 2,3-dinitrotoluene - see attached document for details.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1984
Report date:
1984

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Two male cats were administered either 10 or 50 mg/kg bw of 2,4-DNT (purity = 99%) by
gavage in lutrol.
GLP compliance:
no
Remarks:
Data from collection/ handbook, no informaiton on completion date or compliace is available.
Test type:
other: Two male cats were administered either 10 or 50 mg/kg bw of 2,4-DNT (purity = 99%) by gavage in lutrol.
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-dinitrotoluene
EC Number:
204-450-0
EC Name:
2,4-dinitrotoluene
Cas Number:
121-14-2
Molecular formula:
C7H6N2O4
IUPAC Name:
1-methyl-2,4-dinitrobenzene
Test material form:
solid
Details on test material:
Molecule for read-across studies
Specific details on test material used for the study:
purity = 99%

Test animals

Species:
cat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
not specified

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: lutrol
Details on oral exposure:
Two male cats were administered either 10 or 50 mg/kg bw of 2,4-DNT (purity = 99%) by
gavage in lutrol.
Doses:
10 or 50 mg/kg bw of 2,4-DNT (purity = 99%)
No. of animals per sex per dose:
1
Control animals:
no
Details on study design:
Two male cats were administered either 10 or 50 mg/kg bw of 2,4-DNT (purity = 99%) by
gavage in lutrol.
Statistics:
N/A

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
50 mg/kg bw
Based on:
test mat.
Mortality:
Toxic effects were found at the high dose. The cause of death of the high-dose cat 1 week after treatment was renal
insufficiency.
Clinical signs:
Toxic effects were found at the high dose. Methaemoglobin levels increased
up to 42% within 7 hours after treatment, and then those decreased to basal levels after 48
hours. Nevertheless, Heinz-bodies were noted within 48 hours after treatment (55%).
Other findings:
Methaemoglobin levels increased up to 42% within 7 hours after treatment, and then those decreased to basal levels after 48 hours. Nevertheless, Heinz-bodies were noted within 48 hours after treatment (55%).

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Conclusions:
Based on cat data (death following oral administration of 50 mg/kg b.w.) 2,4-DNT is considered to be classified for acute toxicity as toxic by oral exposure at Category 3.
Executive summary:

Therefore, based on cat data (death following oral administration of 50 mg/kg b.w.) 2,4-DNT

is considered to be classified for acute toxicity as toxic by oral exposure. Since oral and

inhalation absorption values were estimated to be 100% in experimental animals and humans,

it seems reasonable to extrapolate toxicity by inhalation from oral toxicity data and to classify

2,4-DNT as toxic by inhalation. Finally, taking into account that the dermal absorption value

was estimated to be 10% in rodent and by extrapolation in experimental animals and humans,

2,4-DNT should be considered as borderline toxic by dermal exposure.