3-hydroxy-2-methylquinoline-4-carboxylic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

The sutdy was not performed according to GLP. No monitoring analysis was performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-2-methylquinoline-4-carboxylic acid

EC Number:

204-198-1

EC Name:

3-hydroxy-2-methylquinoline-4-carboxylic acid

Cas Number:

117-57-7

Molecular formula:

C11H9NO3

IUPAC Name:

3-hydroxy-2-methylquinoline-4-carboxylic acid

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

Chromophor was used as a vehicle in a concentration of 100 mg/L in the stock solution containing 500 mg/L test substance. By dilution of the stock solutions also the vehicle was diluted accordingly.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Source: Institut National de Recherche Chimique Appliquee, France

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Effects were recorded after 3, 6, 24 and 48h of exposure

Test conditions

Hardness:

2.7 mMol/L

Test temperature:

19-21°C

pH:

8.8

Dissolved oxygen:

>2 mg/L

Salinity:

artificial drinking water

Conductivity:

600-700 µS/cm

Nominal and measured concentrations:

nominal

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Mortality was only observed in the highest test concentration of 500 mg/L. However the author reports that in concentration >125 mg/L the substance precipitated partially. Especially in the 500 mg/L test solution daphnia were adsorbed to particles. Therefore it cannot be excluded that the effects in this concentration were physical effects.

Any other information on results incl. tables

The table shows the number of mobile daphnia after 48h:

concentration	vessel 1	vessel 2	vessel 3	vessel 4
control	5	5	5	5
solvent control	5	5	5	5
500 mg/L	1	0	0	0
250 mg/L	5	5	5	5
125 mg/L	5	5	5	5
62.5 mg/L	5	5	5	5
31.25 mg/L	5	5	5	5

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No effects to daphnia are observed up to the limit of the water solubility.

Executive summary:

In a test according to EU methode C.2 with daphnia magna, immobilisation was only observed in the highest test concentration of 500 mg/L. It is however questionable whether the observations are due to physical effects as the substance was partially precipitated and daphnia were found adsorbed to particles. The EC50 was therefore defined as >250 mg/L. As a conclusion, no effects up to the limit of the water solubility were observed.