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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Test material information:
Composition 1
Analytical monitoring:
yes
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: Pseudokirchneriella subcapitata HINDAK, SAG 61.81
- Source (laboratory, culture collection): Collection of algal cultures in University of Göttingen/Germany

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Details on test conditions:
TEST SYSTEM
- Test vessel:Sterile Erlenmeyer flasks (volume 250 ml; sealed with cotton wool plugs)
- Type (delete if not applicable): closed
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH:no
- Photoperiod: 24 hours/day light
- Light intensity and quality: Approximately 4440 to 8880 lux, corresponding to 60 to 120 μ E*m-2*s-1
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
402 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
116 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
15.4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The study was conducted under static conditions with an initial cell density of 6305 cells/ml. A stock solution of 500 mg/L was freshly prepared with demineralized water and stirred for 15 minutes (1100 rpm, room temperature). Seven concentrations were tested in a geometrical series with a dilution factor of 3.2: 0.466; 1.49; 4.77; 15.3; 48.8; 156.3; 500 mg/L, corresponding to the geometric mean measured test item concentrations: 0.402; 1.43; 4.90; 15.4; 53.3;
178; 558 mg/L. The environmental conditions were within the acceptable limits. The concentrations of the test item in all test concentrations and the control were analytically verified by GC-MS at the start and the end of exposure. The measured concentrations of IPGMA in the fresh media (0 h) were in the range of 104 to 120% of the nominal values. The measured concentrations in the old media (72 h) were in the range of 72 to 108% of the nominal values.

 

Inhibition of Growth Rate

NOEC

15.4

LOEC

53.3

ErC10

116 (89.9 – 135)

ErC20

202 (185 – 324)

ErC50

402 (344 – 455)

 

Inhibition of Yield

NOEC

1.43

LOEC

4.90

EyC10

n.d.

EyC20

34.8 (20.8 – 55.7)

EyC50

125 (104 – 148)

Validity criteria fulfilled:
yes
Conclusions:
In this study, Isoporopylidenglycerolmethacrylate (IPGMA) was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values: Based on the geometric mean measured test item concentrations, the NOEC values (0-72 hours) for inhibition of growth rate and inhibition of yield were 15.4 mg/L and 1.43 mg/L. The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 402 (344 - 455) mg/L and 125 (104 - 148) mg/L, respectively.

Description of key information

In this study, Isopropylidenglycerolmethacrylate (IPGMA) was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values:

Based on the geometric mean measured test item concentrations, the NOEC values (0-72 hours) for inhibition of growth rate and inhibition of yield were 15.4 mg/L and 1.43 mg/L. The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 402 (344 - 455) mg/L and 125 (104 - 148) mg/L, respectively.

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
402 mg/L
EC10, LC10 or NOEC for freshwater algae:
15.4 mg/L

Additional information