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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
guideline study (with GLP and analytics)
Qualifier:
according to
Guideline:
other: EEC Directive 92/32/EEC (December 1992)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
- Concentration of the stock solution: 100 mg/L nominal.
- Eluate: The substance was stirred in M4 medium for about 10 minutes at 22±1°C.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Daphnia magna STRAUS
- Source: Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF.
- Age at study initiation: 2 - 24 hours old
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.2-3.2 mM
Test temperature:
20.1-21.3°C
pH:
8.0
Dissolved oxygen:
8.1-8.5 mg/L
Nominal and measured concentrations:
nominal concentrations: 0, 12.5, 25, 50, 100 mg/L
measured concentrations (nominal concentration of the respective test solution in parenthesis): not detectable (0 mg/L), 10-12 mg/L (12.5 mg/L), 98-100 mg/L (100 mg/L)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 mL glass tubes with flat bottom, 10 mL test volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
M4 medium with the properties:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/L
Ratio Ca/Mg ions: about 4 : 1
pH value: 7.5 - 8.5
Conductivity: 550 - 650 microS/cm

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light (about 2-7 microeinstein/m*m*s (400-750 nm))

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations: mobile daphnids after 0, 3, 6, 24 and 48 h
Measurements: Temperature (after 24 and 48 h) , pH and oxygen content after 0 and 48 h
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: no
Results with reference substance (positive control):
- EC50 (24 h): 1.60 mg/L
Validity criteria fulfilled:
yes
Conclusions:
48-hour LC50 (Daphnia magna): >100 mg/L
Executive summary:

The acute toxicity of the test substance to aquatic invertebrates was studied in accordance with EEC Directive 92/32/EEC. Daphnia magna were exposed to the test substance at concentrations of 12.5, 25, 50, and 100 mg/L. The 48-hour LC50 was >100 mg/L.

Description of key information

48-hour EC50 (Daphnia magna): >100 mg/L; EEC Directive 92/32/EEC; Reliability = 1

Key value for chemical safety assessment

Additional information

EC50/LC50 for freshwater invertebrates: >100 mg/L