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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
guideline study (no analytics, no GLP)
Qualifier:
equivalent or similar to
Guideline:
other: DIN 38 412
GLP compliance:
no
Analytical monitoring:
no
Details on test solutions:
The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria.
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden orfe (Leuciscus idus)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range): 8.4 cm (range 7.5 - 9.3 cm)
- Weight at study initiation (mean and range): 6.0 g (range 4.4 - 7.6 g)
- Feeding during test: Withdrawal of food one day before and during exposure

ACCLIMATION
- Health during acclimation (any mortality observed): 0%
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
2.5 mmol/L
Test temperature:
20 to 21°C
pH:
7.1 to 7.5
Dissolved oxygen:
7.9 to 8.7 mg/L
Nominal and measured concentrations:
0, 1000, 2150, 4640 and 10000 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm), Volume of water: 10 L
- Aeration: Continuous aeration with oil-free air, slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 6 g fish/L test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982
- Alkalinity: pH about 8.0
- Ca/Mg ratio: 4 : 1

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
observations: mortality and symptoms after 1, 4, 24, 48, 72, and 96 h
measurements: pH and oxygen content after 1, 24, 48, 72 and 96 h
Reference substance (positive control):
yes
Remarks:
Chloroacetamide
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 2 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
- Behavioural abnormalities: Symptoms of intoxication: Narcotic-like state, Tumbling [LOEC: 2150 mg/L]
Results with reference substance (positive control):
- LC50: about 31 mg/L (after 48 h)
Validity criteria fulfilled:
yes
Conclusions:
96-hour LC50 (Leuciscus idus): ca. 2200 mg/L
Executive summary:

The acute toxicity of the test substance to fish was studied in a method equivalent to DIN 38 412. Leuciscus idus were exposed to the test substance at concentrations of 1000, 2150, 4640, and 10000 mg/L. The 96-hour LC50 was ca. 2200 mg/L.

Description of key information

96-hour LC50 (Leuciscus idus): ca. 2200 mg/L; DIN 38 412; Reliability = 2

Key value for chemical safety assessment

LC50 for freshwater fish:
2 200 mg/L

Additional information