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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 26-28, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid: flakes
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsubishi-Chemical Foods Corporation; 63281211
- Expiration date of the lot/batch: March 27, 2018
- Purity test date: May 30, 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: below 15 degrees C, in dark well-ventilated space
- Stability under test conditions: stable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: Two samples from each test solution and control were taken prior to addition of alga, and at the end of the test
- Sample storage conditions before analysis: samples were stored at -25 degrees C
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100.03 mg of test substance was added to 1 L glass bottles. Growth medium was added, and the solution placed in an ultrasonic bath at 30 degrees C for 2 hrs. It was then stirred for 2.5 days at room temperature. The test solution was then filtered.
- Eluate: The eluate was used as the highest test concentration. Lower test concentrations were prepared by additional dilutions with growth medium.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University of Gottingen
ACCLIMATION
- Acclimation period: 3 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 degrees C
- pH:
- 7.21-7.70
- Nominal and measured concentrations:
- Due to difficulties analyzing the concentration of test substance in the test medium, nominal concentrations were reported.
The nominal concentrations were 0, 6.25, 12.5, 25.0, 50.0, and 100.0 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass flasks
- Type (delete if not applicable): open (air-permeable silicone-sponge caps)
- Material, size, headspace, fill volume: glass, 250 mL fill volume
- Aeration: No
- Initial cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Light intensity and quality: 92.17 to 93.28 uE m^-2 s^-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter at 24, 48, and 72 hrs
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 0, 6.25, 12.5, 25.0, 50.0, and 100 mg/L
- Results used to determine the conditions for the definitive study: at 100 mg/L the yield was inhibited 45.1% - Reference substance (positive control):
- yes
- Remarks:
- The sensitivity of the test organism is tested twice a year with 3.5-dichlorophenol.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no abnormalities were observed
- Unusual cell shape: no
- Colour differences: no
Any other information on results incl. tables
Inhibition of Algal Growth at 72 hrs
Concentration (mg/L) |
Growth rate (1/d) |
% Inhibition – Growth Rate |
Yield (cells x 104/mL) |
% Inhibition - Yield |
0 |
1.6143 |
-- |
127.4625 |
-- |
6.25 |
1.5476 |
4.13* |
102.8725 |
19.29* |
12.5 |
1.6687 |
-3.37 |
148.5000 |
-16.50 |
25.0 |
1.5926 |
1.35 |
117.8750 |
7.52 |
50.0 |
1.5414 |
4.52* |
100.9725 |
20.78* |
100.0 |
1.5532 |
3.79 |
105.0925 |
17.55 |
* Significantly different from Controls
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hr EC50 based on both growth rate and yield is > 100 mg/L.
- Executive summary:
The toxicity to algae for the test substance LMDT was determined in an OECD guideline 201 test. The nominal concentrations tested were 0, 6.25, 12.5, 25.0, 50.0, and 100.0 mg/L. Due to difficulties in analyzing the substance, analytical concentrations were not determined. The 72-hr EC50 based on both growth rate and yield is > 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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