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EC number: 947-390-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1995
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Remarks:
- Those in vivo data have been considered sufficient, together with QSARs predictions on the main constituant, to classify the registered substance without further testing using a weight of evidence approach.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Identification of New Allergenic Constituents and Proof of Evidence for Coniferyl Benzoate in Balsam of Peru
- Author:
- Hausen
- Year:
- 1 995
- Bibliographic source:
- American Journal of Contact Dermatitis, Vol 6, N o 4 (Decem ber), 1995: p p 199-208
- Reference Type:
- publication
- Title:
- Studies on the sensitizing capacity of imidazole and triazole derivatives. Part lI.
- Author:
- Hausen BM, Angel M
- Year:
- 1 992
- Bibliographic source:
- Am J Contact Dermatitis 3:95-101
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - Principle of test: Sensitization was performed with Cinnamyl Cinamate according to a modified Freund's complete adjuvant technique (Guinea pigs modified FCA method). The method was developed from Freund's complete adjuvant test (FCAT) and the guinea pig maximisation test (GPMT) in order to determine the sensitizing capacity of medium and weak allergens.
- Short description of test conditions:
Induction by 6 intradermal injections on day 1, day 5 and day 9 of Cinnamyl cinnamate dissolved in FCA and physiologicals aline.
Topical challenge on day 20 by open application. The challenge doses were 3 and 10% Cinnayl cinnamate in acetone.
Reading at 24, 48 and 72 hours.
The mean response was computed as the quotient of the sum of all reactions obtained divided by the total number of treated animals. A mean response of 0 to 1 was considered as weak, 1 to 2 as moderate, and greater than 2 as strong.
- Parameters analysed / observed: Skin irritation. Scoring of the response 0 to 1 was considered as weak, 1 to 2 as moderate, and greater than 2 as strong - GLP compliance:
- not specified
- Type of study:
- other: modified Freund's complete adjuvant method
- Justification for non-LLNA method:
- At the time of study completion (1995), the LLNA OECD test method was not adopted.
Test material
- Reference substance name:
- Cinnamyl cinnamate
- EC Number:
- 204-566-1
- EC Name:
- Cinnamyl cinnamate
- Cas Number:
- 122-69-0
- Molecular formula:
- C18H16O2
- IUPAC Name:
- 3-phenylprop-2-enyl 3-phenylprop-2-enoate
- Test material form:
- not specified
- Details on test material:
- - Purchased (ICN, NY)
- Lot/Batch n°: not reported
- Purity: not reported
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- other: Pirbright white strain
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: not reported
- Females (if applicable) nulliparous and non-pregnant: not specified
- Age at study initiation: ot reported
- Weight at study initiation: 280 to 350 g
- Housing: 3 to a cage
- Diet (e.g. ad libitum): yes, pellets
- Water (e.g. ad libitum): yes
- Acclimation period: not reported
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 to 24 °C
- Humidity (%): 50 to 55 %
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): 10 hours light
- IN-LIFE DATES: not reported
Study design: in vivo (non-LLNA)
Induction
- Route:
- intradermal
- Vehicle:
- other: Freund's complete adjuvant (FCA) + physiological saline (1:1)
- Concentration / amount:
- 15 mg of Cinnamyl cinnamate in 8 mL of solvent
- Day(s)/duration:
- 6 intradermal injections on day 1, 5 and 9.
- Adequacy of induction:
- not specified
Challengeopen allclose all
- No.:
- #1
- Route:
- epicutaneous, open
- Vehicle:
- other: acetone
- Concentration / amount:
- 3%
- Day(s)/duration:
- Eleven days after induction
- Adequacy of challenge:
- highest non-irritant concentration
- No.:
- #2
- Route:
- epicutaneous, open
- Vehicle:
- other: acetone
- Concentration / amount:
- 10%
- Day(s)/duration:
- Eleven days after induction
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 10
- Details on study design:
- RANGE FINDING TESTS: determination of irritancy
Ten guinea pigs were treated with the emulsion of FCA and physiological saline but without the tested material. They served also to determine patterns of irritation. One day before challenge, these animals were tested by applying different molar concentrations of test material to the right flank (0.1, 0.3 and 1.0 mol). The results were read after 24 hours.
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: Induction by 6 intradermal injections on day 1, day 5 and day 9 of Cinnamyl cinnamate dissolved in FCA and physiologicals aline.
- Test groups: yes
- Control group: see range finding test
- Concentrations: 15 mg of Cinnamyl cinnamate in 8 mL of solvent
B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: Day 20 (eleven days following induction)
- Exposure period: open application
- Site: clipped and shave flank of the animals
- Concentrations: 3 and 10% in acetone
- Evaluation (hr after challenge): 24, 48 and 72 hours
OTHER:
The mean response was computed as the quotient of the sum of all reactions obtained divided by the total number of treated animals. A mean response of 0 to 1 was considered as weak, 1 to 2 as moderate, and greater than 2 as strong. - Challenge controls:
- Not reported
- Positive control substance(s):
- not specified
Results and discussion
- Positive control results:
- Not applicable
In vivo (non-LLNA)
Resultsopen allclose all
- Key result
- Reading:
- 1st reading
- Group:
- test chemical
- Dose level:
- 3% in acetone
- Total no. in group:
- 10
- Clinical observations:
- not reported
- Remarks on result:
- positive indication of skin sensitisation
- Remarks:
- detailed results not reported
- Key result
- Reading:
- 1st reading
- Group:
- test chemical
- Dose level:
- 10 % in acetone
- Total no. in group:
- 10
- Clinical observations:
- not reported
- Remarks on result:
- positive indication of skin sensitisation
- Remarks:
- detailed results
- Reading:
- 1st reading
- Group:
- negative control
- Dose level:
- Acetone
- No. with + reactions:
- 0
- Total no. in group:
- 0
- Clinical observations:
- not reported
- Remarks on result:
- not measured/tested
- Remarks:
- No negative control reported in the available public data
- Reading:
- 1st reading
- Group:
- positive control
- Dose level:
- Not reported
- No. with + reactions:
- 0
- Total no. in group:
- 0
- Clinical observations:
- not reported
- Remarks on result:
- not measured/tested
- Remarks:
- No positive control reported in the available public data
Any other information on results incl. tables
Mean response at 3 % (24, 48 and 72h after challenge) = 0.10
Mean response at 10 % (24, 48 and 72h after challenge) = 0.47
Cinnamyl cinnamate was concluded as weak sensitiser
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Conclusions:
- Under the test conditions, Cinnamyl cinnamate, the main constituent of the registered substance, is classified as sensitiser category 1 according to Regulation (EC) No. 1272/2008 (CLP) and to the GHS. The registered substance has not been tested itself in appropriate in vitro or in vivo tests but its main constituent Cinnamyl cinnamate is present above the CLP generic concentration limit of 1% that triggers classification of the mixture. Therefore, the registered substance is classified as a skin sensitiser Cat. 1 without further testing according to the Regulation (EC) No 1272/2008.
- Executive summary:
Sensitization was performed with Cinnamyl Cinamate according to a modified Freund's complete adjuvant (FCA) test method. The method was developed from Freund's complete adjuvant test (FCAT) and the guinea pig maximisation test (GPMT) in order to determine the sensitizing capacity of medium and weak allergens.
Ten female albino Guinea pigs of the Pirbright white strain were treated as follows: Induction by 6 intradermal injections on day 1, day 5 and day 9 with Cinnamyl cinnamate dissolved in FCA and physiologicals aline (1:1) (15 mg in 8 mL). Topical challenge on day 20 by open application. The challenge doses were 3 and 10% Cinnamyl cinnamate in acetone. Reading was done at 24, 48 and 72 hours.
The mean response was computed as the quotient of the sum of all reactions obtained divided by the total number of treated animals. A mean response of 0 to 1 was considered as weak, 1 to 2 as moderate, and greater than 2 as strong.
Cutaneous reactions were observed and considered to be sensitisation reactions. Mean responses of 0.1 at 3% and 0.47 at 10% indicate that Cinnamyl cinnamate is a weak sensitiser.
Mortality, body weight gain and clinical signs were not reported.
Under the test conditions, Cinnamyl cinnamate is considered as sensitiser.
The registered substance is a UVCB composed of several constituents and in that, it can be considered as a mixture according to the definition of the CLP Regulation. The decision logic for classification of mixtures from the ECHA Guidance on the Application of the CLP Criteria (2017) was used to determine the skin sensitising potential of the registered substance.
Cinnamyl cinnamate, the main constituent of the registered substance, was considered as a skin sensitiser in a in vivo study. The registered substance has not been tested itself in appropriate in vitro or in vivo tests but its main constituent Cinnamyl cinnamate is present above the CLP generic concentration limit of 1% that triggers classification of the mixture. Therefore, the registered substance is classified as a skin sensitiser Cat. 1 according to the Regulation (EC) No 1272/2008.
Those in vivo data have been considered sufficient, together with QSARs predictions on the main constituant, to classify the registered substance without further testing using a weight of evidence approach.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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