Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 249-648-8 | CAS number: 29461-13-0
Table - Mutagenicity testing of the test chemical in the Salmonella/microsome assay TA100, TA98, TA97a and TA102 tester strains
Number of revertants (Mean±SD)
(–) Dose not tested.
(*) Toxicity apparent as an alteration of the background lawn.
(/+) Mutant counts of individual plates.
Values are the means±SD of three plates of one (out of two) representative experiment.
Salmonella reverse mutation assay was performed to determine the mutagenic nature of the given test chemical on a tester strains of Salmonella typhimurium TA102, TA100, TA98 and TA97 with and without metabolic activation system extracted from Lyophilized rat liver induced by Aroclor 1254 at dose concentrations of 0, 250, 500, 750, 1000, 1250, 1500, 2000, 2500 µg/plate. The Salmonella mutagenicity test was performed by the plate incorporation method. Briefly, 2 ml of top-agar was mixed with 100µl of an overnight grown culture of S. typhimurium, 100µl of the test substance (diluted in ethanol analytical grade, Merck, KGaA), the negative control, or the positive control (PC) and 500µl of the phosphate buffer or the S9 mixture. Ethanol served as the negative (solvent) controls, while the positive control substances were: SA (0.5µg/plate), NPD (1µg/plate), MC (0.5µg/plate), 4-NQNO (1µg/plate), 2AF (10µg/plate), 2AA (0.5 or 1µg/plate), and B-(α)-P (50µg/plate). SA and MC were dissolved in distilled water and dimethyl sulfoxide (DMSO) was used as vehicle for the other positive controls. Plates were incubated at 37°C for 72 h in the dark and then scored for revertant his+ bacteria colonies. Every determination was made in triplicate and each experiment was repeated at least once in order to check the reproducibility of the results. A preliminary test carried out with TA100 strain without and with addition of S9 mixture. The upper limit of the dose interval tested was either the highest non-toxic dose or the lowest toxic dose determined in this preliminary assay. The given test chemical was non toxic at highest doses of 2000–2500µg/plate. No mutagenic effect was observed with the four bacterial tester strains, in the absence as well as in the presence of extrinsic metabolic activation. Hence, the given test chemical is not likely to classify as a gene mutant in vitro.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again