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EC number: 607-858-0 | CAS number: 260781-16-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-05-31 to 1994-06-04
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- GLP study according to OECD 203 (1992): effect concentration based on nominal concentrations, although the amount of test item in old media is <80 % of nominal values; recalculation was done
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- (1992)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 1994-03-16
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples were taken of fresh and the corresponding 24-h old media over the whole test period (96 hours).
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dispersion in water
- Controls: yes, one control - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Length at study initiation at test start: 5.1 cm (SD = 0.2 cm)
- Weight at study initiation at test start: 1.91 g (SD = 0.13 g)
ACCLIMATION
- Acclimation period: from 23 - 31 May 1994
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: commercial trout pellets
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): mortality zero prior to test start
FEEDING DURING TEST
None. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- approximately 100 mg/L as CaCO3
- Test temperature:
- 14 °C
- pH:
- 7.6 - 8.0
- Dissolved oxygen:
- 9.8 - 10.0 mg O2/L
- Nominal and measured concentrations:
- Nominal concentrations: control, 3.2, 5.6, 10, 18, 32 mg/L
Measured concentrations (recalculation of mean measured): control, 1.97, 3.38, 6.64, 12.22, 23.81 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria holding 20 L test media
- Aeration: via narrow bore glass tubes
- Renewal rate of test solution: yes, daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.96 g bodyweight/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16h light: 8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study
- Test concentrations: 1.0 to 100 mg/L with 5 concentrations
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: geometric mean of LC0/LC100 based on recalculation of mean measured concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 12.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Recalculation of arithmetic mean based on geometric mean of measured concentrations of each renewal period
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- 23.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Recalculation of geometric mean of measured concentrations of the first renewal period only (all fish were dead after 24 h)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: Recalculation of arithmetic mean based on geometric mean of measured concentrations of each renewal period
- Details on results:
- - Increased pigmentation at 6.64 mg/L (mean measured)
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- Analysis of the mortality data by the moving average method of Thompson (1947).
- Sublethal observations / clinical signs:
Cumulative Mortality
Mean Measured Concentration* (mg/L)
Cumulative mortality
(initial population: 10)
3h
6h
24h
48h
72h
96h
Control
0
0
0
0
0
0
1.97
0
0
0
0
0
0
3.38
0
0
0
0
0
0
6.64
0
0
0
0
0
0
12.22
0
0
0
0
0
0
23.81
7
10
10
10
10
10
*Recalculation
Behavioural Observations
Mean Measured Concentration*
(mg/L)
Abnormality
Time (hours)
3
6
24
48
72
96
Control
No abnormalities detected
1.97
No abnormalities detected
3.38
No abnormalities detected
6.64
Increased pigmentation
10/10
10/10
10/10
10/10
12.22
Lethargic
Increased pigmentation
10/10
10/10
10/10
10/10
10/10
10/10
10/10
10/10
10/10
10/10
23.81
Moribund
3/3
A/D
A/D
A/D
A/D
A/D
*Recalculation
A/D = All fish dead.
Analytical results for the test item
Samples
Nominal concentration of test item
(mg/L)
Concentration of test item found
(mg/L)
Concentration of test item expressed as % of nominal
0 hours
(fresh media)
Control
3.2
5.6
10
18
32
< L.O.D.
2.52
4.29
8.04
14.2
26.0
< L.O.D.
78.8
76.5
80.4
79.0
81.2
24 hours
(old media)
Control
3.2
5.6
10
18
32
< L.O.D.
0.96
1.69
3.55
7.33
21.8
< L.O.D.
29.9
30.2
35.5
40.7
68.1
24 hours
(fresh media)
Control
3.2
5.6
10
18
< L.O.D.
3.09
5.20
8.90
17.4
< L.O.D.
96.5
92.9
89.0
96.4
48 hours
(old media)
Control
3.2
5.6
10
18
< L.O.D.
1.28
2.16
7.10
8.94
< L.O.D.
40.1
38.6
71.0
49.6
48 hours
(fresh media)
Control
3.2
5.6
10
18
< L.O.D.
2.63
7.07
9.06
16.7
< L.O.D.
82.2
126.2
90.6
92.7
72 hours
(old media)
Control
3.2
5.6
10
18
< L.O.D.
1.96
2.28
4.79
9.40
< L.O.D.
61.1
40.8
47.9
52.2
72 hours
(fresh media)
Control
3.2
5.6
10
18
< L.O.D.
2.87
4.86
8.72
17.3
< L.O.D.
89.7
86.7
87.2
96.2
96 hours
(old media)
Control
3.2
5.6
10
18
< L.O.D.
1.45
2.47
5.12
10.8
< L.O.D.
45.4
44.1
51.2
60.3
L.O.D. = Limit of Detection
Recalculation of mean measured test item concentration (arithmetic mean) based on geometric mean of the measured test item concentrations (fresh media - old media) for each renewal period.
Nominal test concentration
3.2 mg/L
geometric mean
5.6 mg/L
geometric mean
10 mg/L
geometric mean
18 mg/L
geometric mean
32 mg/L
geometric mean
0 h
(fresh media)
2.52
4.29
8.04
14.2
26.0
24 h
(old media)
0.96
1.56
1.69
2.69
3.55
5.34
7.33
10.2
21.8
23.81
24 h
(fresh media)
3.09
5.20
8.90
17.4
all fish dead
48 h
(old media)
1.28
1.99
2.16
3.35
7.10
7.95
8.94
12.47
all fish dead
48 h
(fresh media)
2.63
7.07
9.06
16.7
all fish dead
72 h
(old media)
1.96
2.27
2.28
4.01
4.79
6.59
9.40
12.53
all fish dead
72 h
(fresh media)
2.87
4.86
8.72
17.3
all fish dead
96 h
(old media)
1.45
2.04
2.47
3.46
5.12
6.68
10.8
13.67
all fish dead
geometric mean (24 h)
23.81
arithmetic mean (96 h)
1.97
3.38
6.64
12.22
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study a lethal effect on Oncorhynchus mykiss was observed at a mean measured concentration of 23.8 mg test item/L after 24 hours.
The 96-hour LC50 was determined to be 17.1 mg/L (mean measured), with a corresponding LC0 (96 h) and LC100 (24 h) of 12.2 mg/L and 23.8 mg/L, respectively. - Executive summary:
The acute toxicity of the test item to fish (Oncorhynchus mykiss) was determined under semi-static conditions with daily renewal of the test media over a period of 96 hours according to OECD 203 (1992). Based on result of the range-finding study, 5 nominal concentrations ranging from 3.2 to 32 mg/L were tested. Effect concentrations (LC50, LC0, LC100) were recalculated based on calculation of mean measured test item concentrations. Increased pigmentation was observed at 6.64 mg/L (mean measured) and 100 % mortality was already observed after the first test medium renewal period (0-24 hours) at 23.8 mg/L (mean measured). The 96-hour LC50 was determined to be 17.1 mg/L based on the calculated geometric mean measured concentrations attribute the toxic effect of LC0 (96 h) and LC100 (24 h).
Reference
Description of key information
The test item was found to be toxic to Oncorhychus mykiss. The 96-hour LC50 was determined to be 17.1 mg/L (mean measured), with a corresponding LC0 (96 h) and LC100 (24 h) of 12.2 mg/L and 23.8 mg/L, respectively.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 17.1 mg/L
Additional information
The acute toxicity of the test item to fish (Oncorhynchus mykiss) was determined under semi-static conditions with daily renewal of the test media over a period of 96 h according to OECD 203 (1992). Based on result of the range-finding study, 5 nominal concentrations ranging from 3.2 to 32 mg/L were tested. Effect concentrations (LC50, LC0, LC100) were recalculated based on calculation of mean measured test item concentrations. Increased pigmentation was observed at 6.64 mg/L (mean measured) and 100 % mortality was already observed after the first test medium renewal period (0-24 hours) at 23.8 mg/L (mean measured).The 96-hour LC50 was determined to be 17.1 mg/L based on the calculated geometric mean measured concentrations attribute the toxic effect of LC0 (96 h) and LC100 (24 h).
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