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EC number: 232-577-1 | CAS number: 9001-05-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 August 2010 to 07 October 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to the OECD Guideline but is not in compliance with GLP.
- Justification for type of information:
- Biodegradability studies according to OECD 301 test guidelines have been performed with a wide variety of industrial enzymes. The physico-chemical properties of enzymes including logKow are very similar. They are proteins built up of amino acids and the type, order and number of the amino acids in the polymer differs between enzymes, determining the 3-dimensional structure, the activity and specificity of the individual enzyme type. Because all enzymes are built up of the same amino acids, the physical and chemical characteristics will be very similar for different enzymes. Therefore, industrial enzymes belonging to other enzyme classes were generally found to be readily biodegradable. Similarly, although the biodegradation study with catalase wasn’t conducted GLP, catalase was also found to be readily biodegradable. The weight-of-evidence indicates that enzymes in general are readily biodegradable.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- no
- Remarks:
- .
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: dissolved oxygen
- Details on study design:
- Test Medium Preparation: BOD nutrients were added at the rate of 1 mL each/L deionized water. Inoculum (15 mL) was added to 15 L of mineral medium. The test medium was aerated for approximately 20 minutes.
Preparation of Blank Solution: Mineral media (3.5 L) was used as a blank, and homogenized for approximately 40 minutes.
Preparation of Test Substance Solution (5 mg/L): The test substance (17.6 mg) was added to 3.5 L of test medium, and homogenized for approximately 30 minutes.
Preparation of Reference Solution: Distilled aniline (7.1 mg) was added to 3.5 L of test medium, and homogenized for approximately 30 minutes.
Preparation of Toxicity Reference Solution: 6.9 mg of distilled aniline and 17.8 mg of the test substance were added to 3.5 L of test medium, and homogenized for approximately 35 minutes.
BOD Bottles: Each solution was siphoned from the lowest quarter (not the bottom) of prepared solution container and transferred to labeled BOD bottles. The day 0 samples were examined for dissolved oxygen and the remaining bottles were to be stored in the dark and incubated at 21 °C.
Sampling Intervals: Two BOD bottles for each solution were removed at each sampling interval (0, 7, 14, and 28 days). The oxygen in each duplicate set of solutions was measured and recorded at each interval. - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 64.6
- Sampling time:
- 28 d
- Parameter:
- COD
- Value:
- 0.14 mg O2/g test mat.
- Results with reference substance:
- A value of 67.4% biodegradation was obtained on Day 14 for distilled aniline.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the study conditions, Catalase is considered to be readily biodegradable.
- Executive summary:
The study was conducted to measure the biodegradability of Catalase according to the OECD Guideline 301-D in an aerobic, aqueous medium at a concentration of approximately 5 mg/L of active material.
The solutions were prepared using deionized water with activated sludge. The solutions were kept in closed bottles in the dark at 21°C. The degradation was measured by dissolved oxygen analyses over a 28-day period. A standard reagent of known performance (distilled aniline) and a blank were tested at the same time to serve as a control for biological activity.
Under the condition of the study, maximum means of 29.1 %, 55.4% and 64.6% biodegradation were obtained for the test substance on Days 7, 14, and 28, respectively. Therefore, the test substance is considered to be readily biodegradable.
Reference
Table 1: Dissolved Oxygen Measurements
Test Group |
Day0 DO (mg/L) |
Day7 DO (mg/L) |
Day14 DO (mg/L) |
Day 28 DO (mg/L) |
Blank |
8.18 |
7.83 |
7.71 |
7.55 |
8.19 |
7.88 |
7.77 |
7.46 |
|
Test Substance |
8.41 |
7.88 |
7.56 |
7.19 |
8.42 |
7.88 |
7.60 |
7.37 |
|
Distilled Aniline |
8.30 |
7.01 |
4.50 |
4.16 |
8.25 |
7.57 |
4.59 |
4.16 |
|
Toxicity Reference |
8.36 |
5.14 |
4.36 |
*measurements did not take place |
8.39 |
5.05 |
4.21 |
Table 2: Biodegradation Calculations:
|
Day 7 calculations |
Day 14 calculations |
Day 28 calculations |
Blank Depletion (mgIL) |
0.33 |
0.445 |
0.68 |
Distilled Aniline Net D.O. Depletion (mg/L) |
0.655 |
3.285 |
3.435 |
Distilled Aniline ThOD (mg02/mg) |
2.405 |
||
Distilled Aniline Dose (mg/L) |
2.028 |
||
Distilled Aniline Calculated % degradation |
13.4% |
67.4% |
70.4% |
Test Substance Net D.O. Depletion (mg/L) |
0.205 |
0.39 |
0.455 |
Test Substance COD (mgO2/mg) |
0.14 |
||
Test Substance Dose (mg/L) |
5.03 |
||
Test Substance Calculated % Degradation |
29.1% |
55.4% |
64.6% |
Description of key information
Catalase was found to be readily biodegradable in the OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The study was conducted to measure the biodegradability of Catalase according to the OECD Guideline 301-D in an aerobic, aqueous medium at a concentration of approximately 5 mg/L of active material. The solutions were prepared using deionized water with activated sludge. The solutions were kept in closed bottles in the dark at 21°C. The degradation was measured by dissolved oxygen analyses over a 28-day period. A standard reagent of known performance (distilled aniline) and a blank were tested at the same time to serve as a control for biological activity. Under the condition of the study, maximum means of 29.1 %, 55.4% and 64.6% biodegradation were obtained for the test substance on Days 7, 14, and 28, respectively. Therefore, the test substance is considered to be readily biodegradable.
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