Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-029-7 | CAS number: 2978-58-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-02-22 to 2017-04-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - TOC value of the test substance: 666 mg/g
- Purity: 99.2 area %
- Batch Nr.:85603856P0
- Homogeneity: homogeneous
- Physical state / appearance: liquid / colorless, clear
- Density: 0,8168 g/mL at 23 °C - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany was collected on 27 February 2017 from the aeration tank of the plant. A suitable aliquot of the suspension was sieved by a finely woven mesh with a mesh size of about 1 mm. To reduce the content of inorganic carbon in the blank control the activated sludge suspension was aerated with pressured air for about 48 hours at 22 ± 2° C. At the day of exposure, the aeration was stopped and the sludge was allowed to settle. The supernatant was discarded and the residue of the sludge suspension was filled up with drinking water. The sludge of the suspension was adjusted to a concentration 800 mg/L of dry weight. Aliquots of 0.5 mL were added to the test vessels to obtain a sludge
concentration of 4 mg/L dry substance in the test assays. Reason for the choice of the test system: The test guideline recommends the usage of activated sludge from a wastewater treatment plant, treating municipal sewage. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- 29 blank control assays
31 test substance assays
29 inhibition control test assays
17 reference substance assays
5 assays for abiotic control
The pH value of the mineral medium was checked and adjusted to 7.4 with 1 molar sulphuric acid. The CO2-Headspace Test was performed in 160 mL incubation bottles filled up to a volume of 100 mL. The oxygen, available in the headspace of the bottles, is used for the aerobic mineralization processes. The amount of oxygen allows the complete biodegradation. The aqueous phase, a mineral medium with municipal inoculum contains the test compound, directly added by a suitable micro syringe. The bottles were closed with butyl rubber septa gas tight and shaken by an orbital shaker with a speed of about 150 r/min and incubated at a temperature of 22 ± 2 °C. - Reference substance:
- aniline
- Preliminary study:
- Yes. A pH- shift in the test vessels, induced by addition of the test substance to the test medium was checked in a preliminary test. No shift of the pH-value was detected. The pH-value from the mineral test medium was therefore assumed for the test
assays at start of exposure. This pH-value of the mineral medium after correction was 7.4. - Test performance:
- All validity criteria were met and no deviations from the test guidelines or other incidents occurred during the course of the reported test which may have influenced.
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 1
- Sampling time:
- 28 d
- Results with reference substance:
- - biodegradation: 82 % after 14 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
Reference
Nominal TOC [mg/L] concentration in the test assays: RS : 20.0, IH: 40.0, PC: 20.0, TS: 20.0. Abbreviations: TS = test substance assay, RS = reference substance assay, IH = inhibition control assay, PC = abiotic control assay, TOC = total organic carbon, mv = mean value.
|
Degree of biodegradation [%] |
|||||||||||
Test duration [days] |
RS1 |
RS2 |
RS3 |
R mv |
IH1 |
IH2 |
IH3 |
IH mv |
TS1 |
TS2 |
TS3 |
TS mv |
2 |
2 |
2 |
2 |
2 |
2 |
4 |
1 |
2 |
0 |
1 |
1 |
1 |
5 |
57 |
58 |
58 |
58 |
28 |
28 |
28 |
28 |
0 |
0 |
-1 |
0 |
8 |
76 |
78 |
75 |
76 |
42 |
38 |
40 |
40 |
1 |
1 |
1 |
1 |
12 |
81 |
79 |
81 |
80 |
40 |
39 |
38 |
39 |
0 |
0 |
-1 |
0 |
14 |
84 |
83 |
79 |
82 |
39 |
43 |
43 |
42 |
1 |
1 |
1 |
1 |
19 |
40 |
40 |
40 |
40 |
1 |
0 |
0 |
0 |
||||
23 |
42 |
43 |
45 |
43 |
0 |
0 |
1 |
0 |
||||
26 |
43 |
42 |
42 |
42 |
0 |
0 |
0 |
0 |
||||
Test duration [days] |
PC1 |
PC2 |
PC3 |
P mv |
IH1 |
IH2 |
IH3 |
IH mv |
TS1 |
TS2 |
TS3 |
TS4 |
28 |
-1 |
-1 |
-2 |
-1 |
40 |
40 |
42 |
41 |
-1 |
0 |
0 |
1 |
TS5 |
TS mv |
|||||||||||
0 |
0 |
Description of key information
not readily biodegradable
Key value for chemical safety assessment
Additional information
The ready biodegradability of the test substance was investigated under GLP conditions by means of a CO2 Headspace Test, according to OECD guideline 310. The biodegradation of the test substance was followed by exposing it to microorganisms of non-adapted activated sludge from a municipal wastewater treatment plant. An inoculum control, containing sole pretreated activated sludge, a functional control with the reference substance aniline as well as a toxicity control, including both test item and reference item, was conducted simultaneously. The test concentration selected as appropriate was 20 mg/L TOC, corresponding to approximately 30 mg/L test substance. The flasks were incubated for 28 days at 22 ± 2 °C under aerobic and dark conditions. The biodegradability was derived from TIC analysis of the quantity of CO2 produced within the test period by respiration of bacteria. The mean value of degree of biodegradation was calculated by the single values of five test assays at the end of exposure with a limit of confidence of 95 %.
In result, the required pass level for ready biodegradability was not reached. The degree of biodegradation after an exposure period of 28 days was 0 ± 1 % TIC/TOC. As proven by the toxicity control, no inhibitory effects on microorganisms is expected at the tested concentration of 20 mg/L (degree of biodegradation after 28 days: 41 %).
In conclusion, the test item is not regarded to be in the 10-d-window and after 28 days readily biodegradable. Based on determined rate of biodegradation at the end of exposure the test item can be evaluated as poorly biodegradable in this test. All validity criteria were met and no deviations from the test guidelines or other incidents occurred during the course of the reported test which may have influenced. Based on this result, the test substance has to be regarded as not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.