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EC number: 203-291-4 | CAS number: 105-37-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-11-18 to 2017-04-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- The read across justification is included as attachment to section 13 in the IUCLID dataset.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.00, 2.50, 5.00, 10.0, 20.0 and 40.0 mg/L (nominal)
- Sample storage conditions before analysis: in the refrigerator at 6 +/- 2°C - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Appropriate volumes of the test item were pipetted directly into the test vessels filled with appropriate volumes of dilution water. The test media were mixed with an ultraturrax (1 min, 12000 rpm). This procedure was repeated for every water renewal. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: single brood stock (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin)
- Length at study initiation (length definition, mean, range and SD): mean: 2.76 cm
- Weight at study initiation (mean and range, SD): mean: 0.19 g
- Feeding during test: no
ACCLIMATION
- Acclimation period: at least 12 d
- Acclimation conditions (same as test or not): Same as test.
- Type and amount of food: Sera Vipan, 4% of fish body weight per feeding day
- Feeding frequency: 3 times/week
- Health during acclimation (any mortality observed): No mortality observed. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 65 mg CaCO3/L
- Test temperature:
- 22.6 - 23.0°C
- pH:
- 7.14 - 7.77
- Dissolved oxygen:
- 81 - 100%
- Nominal and measured concentrations:
- Nominal: 0, 2.50, 5.00, 10.0, 20.0 and 40.0 mg/L
Measured: < LOQ, 1.31, 2.84, 5.25, 8.66 and 18.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 13 L were used, filled with approximately 10 L of dilution water (dimensions: 25.5/18/28 cm, depth of water: 20 cm) and covered with a floating lid (e.g. with aluminium foil), to reduce the loss of the test item.
- Type (delete if not applicable): semi-static (covered with aluminium foil)
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: Fish density: 0.131 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin
- Chlorine: filtered on activated charcoal and aerated for at least 24 hours to remove chlorine
- Hardness: 10 - 250 mg CaCO3/L
- pH: 6.0 - 8.5
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours/light
- Light intensity: mean value: 116 Lux.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.) after 3, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: threshold test with 16 mg/L (29 % mortality); complete response test. 0, 2.50, 5.00, 10.0, 20.0 and 40.0 mg/L (nominal)
- Results used to determine the conditions for the definitive study: yes, full test with 5 test concentrations. - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.74 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: calculated as mean from the values of LC0 and LC100
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9.04 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 5.25 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 8.66 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: At a concentration level of 8.66 mg/L and 18.9 mg/L the fishes showed lethargy, took side positions, were staggering and were hyperventilating.
- Mortality of control: no
- Other adverse effects control: no - Sublethal observations / clinical signs:
Table 1: Cumulative mortality (%) (n=7)
Geometric mean measured concentration (mg/L)
Cumulative mortality (%) after different exposure periods (hours)
3
24
48
72
96
18.9
0
100
100
100
100
8.66
0
0
14
100
100
5.25
0
0
0
0
0
2.84
0
0
0
0
0
1.31
0
0
0
0
0
Control
0
0
0
0
0
Table 2: Environmental conditions
Exposure time (days)
Geometric mean measured concentration (mg/L)
Mean oxygen saturation (%)
pH value
Temperature (°C)
0
18.9
100
7.67
22.8
8.66
100
7.71
22.8
5.25
100
7.72
22.7
2.84
100
7.77
22.8
1.31
100
7.75
22.8
Control
100
7.70
22.7
1
18.9
90
7.61
22.9
8.66
94
7.64
22.9
5.25
93
7.63
23.0
2.84
94
7.60
23.0
1.31
92
7.58
22.9
Control
93
7.53
22.9
2
18.9
-
-
-
8.66
91
7.66
22.8
5.25
92
7.64
22.8
2.84
93
7.62
22.8
1.31
93
7.61
22.8
Control
94
7.51
22.9
3
18.9
-
-
-
8.66
87
7.40
22.8
5.25
93
7.48
22.8
2.84
93
7.45
22.9
1.31
93
7.43
22.7
Control
94
7.33
22.8
4
18.9
-
-
-
8.66
-
-
-
5.25
82
7.44
22.7
2.84
88
7.53
22.6
1.31
88
7.53
22.8
Control
88
7.55
22.7
- no measurements due to 100% mortality
Table 3: Measured Concentrations and Percent of the Nominal Concentrations of the Test Item
Sampling date
2017-02-20
0 h
(Fresh medium)
2017-02-21
24 h
(Old medium)
2017-02-23
72 h
(Fresh medium)
2017-02-24
96 h
(Old medium)
Nominal concentration of the test item
[mg/L]
Meas. concentration
[mg/L]
%
Meas. concentration
[mg/L]
%
Meas. concentration
[mg/L]
%
Meas. concentration
[mg/L]
%
Geometric mean measured concentration
[mg/L]
40.0
24.2
61
14.7
37
1)
18.9
20.0
11.8
59
6.37
32
1)
8.66
10.0
7.75
78
4.40
44
6.26
63
3.56
36
5.25
5.00
3.45
69
2.47
49
3.23
65
2.36
47
2.84
2.50
1.57
63
1.05
42
1.54
62
1.18
47
1.31
Control
< LOQ
< LOQ
< LOQ
< LOQ
< LOQ
Meas. concentration = Measured concentration of the test item, enrichment factor taken into account
% = Percent of nominal concentration of the test item
LOQ = Limit of Quantification (0.2 mg/L test item)
1) = Not analyzed due to 100% mortality after 24 hours
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 (96 h): 6.74 mg/L based on mortality of Danio rerio (OECD 203).
The toxicity of Ethyl hexanoate to fish (Danio rerio, Zebrafish) was determined in a GLP study according to OECD 203. The LC50 of 6.74 mg/L was calculated from the geometric mean measured values of the LC0 and the LC100. - Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- The read across justification is included as attachment to section 13 in the IUCLID dataset.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.74 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: Source substance
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.77 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: Target substance
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: recalculated value to correct differences in molecular weight between source (MW = 144.2114 g/mol) and target (MW = 102.132 g/mol).
- Conclusions:
- The toxicity of Ethyl propionate to fish (Danio rerio) was predicted from the source substance Ethyl hexanoate (CAS no. 123-66-0) (see read across justification). The effect value (mortality; LC50) was predicted to be 6.74 mg/L. The recalculated value to correct differences in molecular weight between source (MW = 144.2114 g/mol) and target (MW = 102.132 g/mol) was 4.77 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1978
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with national standard methods but insufficient documented. Publication reports only 48 hour effect values for fish, which are not comparable to 96 hours effect values. Further, no details about the test design is reported.
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 L15
- Deviations:
- yes
- Remarks:
- 48 h instead of 96 h tested
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Leuciscus idus melanotus
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Testing laboratory 1
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 113 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Testing laboratory 2
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 89 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Testing laboratory 1
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 89 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Testing laboratory 2
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Testing laboratory 1
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Testing laboratory 2
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The results of a short-term toxicity test with Leuciscus idus melanotus was described in the publication of Juhnke and Lüdemann (1978). In both laboratories the LC50 values were estimated to be 125 mg/L and 113 mg/L, respectively.
Referenceopen allclose all
Description of key information
LC50 (96) = 4.77 mg/L (Danio rerio, OECD 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4.77 mg/L
Additional information
To assess the toxicity of the test substance to fish one study is available. Since this study is not reliable (the test duration was only 48 h) a read-across approach to Ethyl hexanoate (CAS no. 123 -66 -0) was used to cover this endpoint.
Both substances have a similar chemical structure and corresponding physicochemical properties. In addition they show similarity in their environmental fate such as biodegradability and in their ecotoxicity effects, whereby Ethyl hexanoate is more toxic to aquatic organisms than Ethyl propionate. This is especially reflected in the lower LC50 (48 h) value of 9.04 mg/L when Ethyl hexanoate is tested on Danio rerio compared with a higher LC50 (48 h) of > 100 mg/L when Ethyl propionate is tested on Leuciscus idus melanotus (see also supporting study). Ethyl hexanoate can therefore be considered as worst case and is proposed suitable to predict the short-term toxicity to fish of the target substance Ethyl propionate.
The toxicity of the read-across substance to fish was determined in a semi-static test performed according to OECD 203 and GLP. Individuals of Danio rerio were exposed to nominal test concentrations of 2.5, 5.0, 10, 20 and 40 mg/L, equivalent to geometric mean measured concentrations of 1.31, 2.84, 5.25, 8.66 and 18.9 mg/L. After 96 h 100% mortality was observed at 8.66 mg/L whereas 0% mortality was recorded at 5.25 mg/L. The LC50 (96 h) was determined to be 6.74 mg/L..
The determined LC50 valuer of 6.74 mg/L for the source substance was correct for the target substance by taking into account the difference in molecular weight between source (MW = 144.2114 g/mol) and target (MW = 102.132 g/mol) substance. For the target substance a LC50 (96 h) of 4.77 mg/L was calculated.
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