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Environmental fate & pathways

Hydrolysis

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Reference
Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 111 (Hydrolysis as a Function of pH)
GLP compliance:
yes (incl. certificate)
Transformation products:
no
Remarks on result:
not determinable

Pre-testings:

Mixtures of the test item with different buffer solutions were prepared. Due to the fact that the test item was insoluble in the buffer solutions the test item was dissolved additionally in acetonitrile as a solubilizer. Therefore 225.13 mg of the test item were dissolved in 50 ml acetonitrile. The test item is completely soluble in acetonitrile (clear and colourless solution). After that mixtures of the test item with different buffer solutions using 1 % of the solubilizer solution (1.0 ml) were prepared. Therefore 1.0 ml of this solution was added into a 100 ml flask and then filled up with each buffer solution (10 min under

ultrasonication). After that the preparations were visually checked at room temperature (23 °C).

Weight of test item

 

[mg]

Solubilizer used

Volume of test item dissolved in the solubilizer added

[ml]

pH of buffer solution used

Complete Volume

 

[ml]

Observation

63.92

-

 

4.00

50

test item floated at the surface of the turbid solution

65.40

-

 

7.00

50

58.63

-

 

9.00

50

225.13

Acetonitrile (50 ml)

1.0

4.00

100

slightly turbid solution; drops of the test item at the surface of the solution

225.13

Acetonitrile (50 ml)

1.0

7.00

100

225.13

Acetonitrile (50 ml)

1.0

9.00

100

Description of key information

Because of the low solubility in the buffer solutions, the determination of the hydrolysis as a function of pH according to OECD Guideline for the Testing of Chemicals 111 is not feasible and sensible as well.

Key value for chemical safety assessment

Additional information