Sodium 2-[[4-[3-(4-chlorophenyl)-4,5-dihydro-1H-pyrazol-1-yl]phenyl]sulphonyl]ethanesulphonate

Endpoint summary

Description of key information

Short-term toxicity to fish

LC50 (96h): 41 mg/l (nominal)

Short-term toxicity to aquatic invertebrates

EC50 > 100 mg/l nominal concentration

Toxicity to aquatic plants other algae

ErC50 > 100 mg/l based on nominal concentration (corresponding to 0.016 mg/l measured)

Additional information

Short-term toxicity to fish

The acute lethal toxicity of Fluorescent Brightener 312 to Danio rerio and Poecilia reticulata was investigated in two different experiments, both conductedunder static exposure conditions and over a period of 96 h.

10 Danio rerio fish were exposed to each one of the follwoing nominal dose levels: 10, 18, 32, 58 and 100 mg/l. The lower test concentration causing 100 % of mortality was 58 mg/l, while the highest test concentration causing no mortality was 18 mg/l. The LC50 was estimated to be 41 mg/l.

The applied nominal test concentrations in the case of Poecilia reticulata were 1, 10 and 100 mg/l. During the test, all fish were dead at the nominal concentration of 100 mg/l after 48 h of exposure. No mortality was observed at 10 and 1 mg/l and in the control after 96 h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed. Based on these data the nominal lethal concentration (LC50) of test item was estimated to be 10-100 mg/l.

In both the cases, no chemical analysis of the test substance concentrations was conducted. In the first mentioned study, concentration-dependent slight haze and bluish opalescent shimmer were observed in all assays; in the test concentrations 32 - 100 mg/l, a slight to strong foaming occurred and after 24 hours a slight concentration-dependent substance precipitate at the edge of the aquaria was found from the test concentration 32 mg/l.

The test material resulted to be not completely dissolved also in the case of the other experiment.

Short-term toxicity to aquatic invertebrates

No data is available on the target substance for this reason data on similar substance 01 was taken into account.

The substance is highly insoluble in water, therefore this study should have been waived. However, a non-GLP preliminary experiment was carried out at saturation concentration to determine the approximate toxicity of the test item. For preparation of the test solution a supersaturated solution (100 mg/L nominal loading).Based on the results of this non-GLP preliminary test, the test item had no toxic effect on Daphnia magna at aquatic saturation. The 48h EC50, and NOEC values can be determined to be higher than saturation.

Toxicity to aquatic plants other algae

No information on the target substance is available for this reason data on similar substance 01 was taken into account.

The substance was tested onLemna Gibbaaccording to the OECD guideline 221. A limit test was conducted at a single concentration at saturation (corresponding to 100 mg/L nominal concentration) using concurrent control ran. The exposure concentration of the saturated test solution was analytically determined to be 0.016 mg/L (calculated as the geometric mean of start and end concentrations measured at each renewal period).

The plants were exposed to the test substance for 7 days in a semi-static system (24-h water renewal periods).

In this 168-hour semi-static growth inhibition test onLemna gibbathe test item Fluorescent Brightener 313 had no toxic effect at aquatic saturation (measured value: 0.016 mg/L; equivalent to 100 mg/L nominal concentration).

Accordingly, the 168-h EC10, EC20, EC50 and the LOEC values based on growth rate and yield were determined to be > 0.016 mg/L (equivalent to 100 mg/L nominal concentration). The 168-h NOEC values were determined to be 0.016 mg/L (equivalent to 100 mg/L nominal concentration).

All validity criteria were met.

Based on the information available, the substance shows slight toxic effect on fish for this reason it was classified as Aquatic Chronic 3, according to the CLP Regulation (EC) No 1272/2008.