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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
(1992)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on sampling:
- Concentration of 10000 mg/L and control were measured at 24 and 48 hours.
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia mangna STRAUS parthenogenetic females, strain of the Federal Health Office Berlin
- Starting strain: Clone breeding the Federal Health Office Berlin
- Test animals: laboratory breeding of Bayer AG Leverkusen
- Age of test aminmals: 0 - 24 hours
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
13.8 °dH
Test temperature:
20.4 - 20.6 °C after 48 hours
pH:
7.8 - 7.9 after 48 hours
Dissolved oxygen:
9.9 - 10.0 mg O2/L after 48 hours
Nominal and measured concentrations:
10000 mg/L (nominal) plus control
Details on test conditions:
- Test method: cylindrical test vessels, diameter 4.0 cm, height 6.5 cm; 20 mL test medium; not aerated
- Application method: direct weighing
- 10 neonates per vessel, 2 replicates per concentration/control
- The weighed substance in water was used to prepare single test approach 1 hour in an ultrasonic bath treated and then for 24 hours stirred on a magnetic stirrer.
- Undissolved percentage substance were determined by a pleated filter (pore size 7-12 microns) filtered off.
- Criteria of effects: item-induced alteration of the normal mobility and the loss of locomotory actions of the neonates, observed at 24 and 48 hours.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

No inmobilisation and abnormalities (abnormal coloration or abnormal swimming behavior) were observed at 24 and 48 hours and any concentrations.

Validity criteria fulfilled:
not specified
Conclusions:
After 48 hours of exposure no effects against daphnia were observed up to a nominal concentration of 10.000 mg/L
Executive summary:

The acute toxicity to invertebrates of Solvent Violet 59 was determined according to the EU method C.2 "Acute Toxicity for Daphnia" (1992). Daphnia magna was exposed to the test solution of one nominal concentration of Solvent Violet 59 (10000 mg/L) and blank control solution for a period of 48 hours under static conditions. No toxic effect and abnormal behavior were observed. This toxicity study is classified acceptable and satisfies the guideline requirement for the acute daphnia.

Description of key information

After 48 hours of exposure no effects against daphnia were observed up to a nominal concentration of 10.000 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
10 000 mg/L

Additional information

"Should read: EC0 ≥ 10000 mg/L"