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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
(2008)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
(1992)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Name: Zebra fish (Danio rerio)
- Source: West Aquarium, 3422 Bad Lauterberg (Germany)
- Date supplied: 1990-12-19
- Acclimatisation: Stock held since 1990-09-28 and acclimatised to the test conditions since then.
- Temperature: 22 +/- 1 °C
- Dissolved oxygen: >= 80 % of the degree of saturation
- Feeding: twice daily ad libitum (Tetra Min, Tetra Werke, Melle, Germany)
- Mortalities during acclimatisation period: < 5 %
- Medication: none
- Mean standard length (n = 10): 2.9 cm (S.D. = 0.27 cm)
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
No data
Test temperature:
21.3 -23.0 °C measured at each test vessel at the beginning and the end of test
pH:
7.8 - 8.2 measured at each test vessel at the beginning and the end of test
Dissolved oxygen:
7.3 - 8.7 mg O2/L with 95.0 - 100.8 % O2 saturation measured at each test vessel at the beginning and the end of test
Salinity:
n.a.
Nominal and measured concentrations:
10 mg/L (nominal) plus control
All results are expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 0.4 % to 2.0 % of nominal values in the freshly prepared medium and from 0.1 % to 0.8 % of nominal values in the medium after 24 hours of exposure.
Details on test conditions:
TEST METHOD:
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
- Experimental design: 1 test concentration plus 1 control; 7 fish per test concentration; no feeding during the exposure period; semi-static
- Method of initiation: fish were placed in prepared media
- Loading: 0.48 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Temperature: 21.2 to 22.0 °C
- Aeration: gentle aeration via narrow glass tubes
- Test item concentration: 10 mg/L
- Method of administration: direct weighing
- Medium renewal: daily
- Duration of exposure: 96 hours
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

No toxic effects against fish were observed at the limit of water solubility under exposure conditions.

Validity criteria fulfilled:
yes
Remarks:
(The mortality in the controls did not exceed 10 % by the end of the test. - The dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period. - The pH did not vary by more than 1 unit)
Conclusions:
After 96 hours of exposure no effects against fish were observed up to a concentration of 500 mg/L.
Executive summary:

The method described in the Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992) assesses the acute toxic effects (mortality) of various concentrations to a freshwater fish species. The purpose of this method was to determine that concentration which causes a 50 % mortality rate (= LC 50) or, if conducted as a limit test, to determine the acute toxic effects at a maximum test concentration of 10.0 mg/L or at the limit of water solubility. An algal growth inhibition test preceded the fish test. It provided information about the concentration which was used in the fish test. The following nominal concentrations of Solvent Violet 59 were tested in the algal growth inhibition range-finding test: 1.0 and 100.0 mg/L. Because of the low solubility of lower than 1 mg/L fish were exposed in the main test to a limit test concentration of nominally 500 mg/L for a period of 96 hours. As no toxic effects against fish were observed, no statistical analysis was required to determine the LC 50. Additionally any abnormal behaviour or appearance of the fish was reported every 24 hours.

After 96 hours of exposure a LC50 of > 500 mg/L was determined. No toxic effects against fish were observed at the limit of water solubility under exposure conditions. This toxicity study is classified acceptable and satisfies the guideline requirement for the acute fish.

Description of key information

After 96 hours of exposure no effects against fish were observed up to a concentration of 500 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
500 mg/L

Additional information

"Should read as LC50 > 500 mg/L"