Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 229-066-0 | CAS number: 6408-72-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- (2008)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- (1992)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Name: Zebra fish (Danio rerio)
- Source: West Aquarium, 3422 Bad Lauterberg (Germany)
- Date supplied: 1990-12-19
- Acclimatisation: Stock held since 1990-09-28 and acclimatised to the test conditions since then.
- Temperature: 22 +/- 1 °C
- Dissolved oxygen: >= 80 % of the degree of saturation
- Feeding: twice daily ad libitum (Tetra Min, Tetra Werke, Melle, Germany)
- Mortalities during acclimatisation period: < 5 %
- Medication: none
- Mean standard length (n = 10): 2.9 cm (S.D. = 0.27 cm) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- No data
- Test temperature:
- 21.3 -23.0 °C measured at each test vessel at the beginning and the end of test
- pH:
- 7.8 - 8.2 measured at each test vessel at the beginning and the end of test
- Dissolved oxygen:
- 7.3 - 8.7 mg O2/L with 95.0 - 100.8 % O2 saturation measured at each test vessel at the beginning and the end of test
- Salinity:
- n.a.
- Nominal and measured concentrations:
- 10 mg/L (nominal) plus control
All results are expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 0.4 % to 2.0 % of nominal values in the freshly prepared medium and from 0.1 % to 0.8 % of nominal values in the medium after 24 hours of exposure. - Details on test conditions:
- TEST METHOD:
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
- Experimental design: 1 test concentration plus 1 control; 7 fish per test concentration; no feeding during the exposure period; semi-static
- Method of initiation: fish were placed in prepared media
- Loading: 0.48 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Temperature: 21.2 to 22.0 °C
- Aeration: gentle aeration via narrow glass tubes
- Test item concentration: 10 mg/L
- Method of administration: direct weighing
- Medium renewal: daily
- Duration of exposure: 96 hours
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
No toxic effects against fish were observed at the limit of water solubility under exposure conditions.
- Validity criteria fulfilled:
- yes
- Remarks:
- (The mortality in the controls did not exceed 10 % by the end of the test. - The dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period. - The pH did not vary by more than 1 unit)
- Conclusions:
- After 96 hours of exposure no effects against fish were observed up to a concentration of 500 mg/L.
- Executive summary:
The method described in the Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992) assesses the acute toxic effects (mortality) of various concentrations to a freshwater fish species. The purpose of this method was to determine that concentration which causes a 50 % mortality rate (= LC 50) or, if conducted as a limit test, to determine the acute toxic effects at a maximum test concentration of 10.0 mg/L or at the limit of water solubility. An algal growth inhibition test preceded the fish test. It provided information about the concentration which was used in the fish test. The following nominal concentrations of Solvent Violet 59 were tested in the algal growth inhibition range-finding test: 1.0 and 100.0 mg/L. Because of the low solubility of lower than 1 mg/L fish were exposed in the main test to a limit test concentration of nominally 500 mg/L for a period of 96 hours. As no toxic effects against fish were observed, no statistical analysis was required to determine the LC 50. Additionally any abnormal behaviour or appearance of the fish was reported every 24 hours.
After 96 hours of exposure a LC50 of > 500 mg/L was determined. No toxic effects against fish were observed at the limit of water solubility under exposure conditions. This toxicity study is classified acceptable and satisfies the guideline requirement for the acute fish.
Reference
Description of key information
After 96 hours of exposure no effects against fish were observed up to a concentration of 500 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 500 mg/L
Additional information
"Should read as LC50 > 500 mg/L"
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.