Hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 October 2007 to 28 November 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: test according to the guideline under GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 667 mg/L
- Sampling method: sample from the freshly prepared test media and pooled sample at 48 hours at 0 and 667 mg/L (at 667 mg/L analysed in duplicate)
- Sample storage conditions before analysis: frozen at < -10°C

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION as such in reconstituted water
- Controls: reconstituted water

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss
- Source: ECT Oekotoxikologie GmbH, Böttgerstr. 2 - 14, 65439 Flörsheim am Main, Germany.
- Age at study initiation: 6.25 to 15.25 hours
- Method of breeding: conditions similar to test conditions (fed Tetramin once weekly)
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg/L as CaC03

Test temperature:

19-20 °C

pH:

7.6

Dissolved oxygen:

8.2-8.7 mg/L (90-96% of saturation)

Salinity:

N/A

Nominal and measured concentrations:

nominal 0 and 677 mg/L
measured: at 0 and 48 h measured a.i.: 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass beaker filled with 80 mL test medium
- Type: closed
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
In deionised water (conductivity < 5 uScm-l) analytical grade salts were added at the following nominal concentrations:
CaC12 X 2H20 2.0 mmol/L (= 294 mg/L)
MgS04 X 7H20 0.5 mmol/L (= 123 mg/L)
NaHC03 0.75 mmol/L (= 65 mg/L)
KC1 0.075 mmol/L (= 5.8 mg/L)
Water Hardness : 2.5 mmol/L (= 250 mg/L) as CaC03
Alkalinity : 0.8 mmol/L
Ratio of Ca : Mg = 4 : 1 (based on molarity)
Ratio of Na : K = 10 : 1 (based on molarity)

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours light
- Light intensity: 90-150 lux

EFFECT PARAMETERS MEASURED : immobility at 24 and 48 hours

TEST CONCENTRATIONS: 0 and 667 mg/L (based on non-GLP rang finding study)

Reference substance (positive control):

yes

Remarks:

KOH tested twice yearly

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 667 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

measured concentration is 105% of nominal
mortality: 0% in controls , 35% at 667 mg/L

Results with reference substance (positive control):

48 h EC50 1.6 mg/L

Reported statistics and error estimates:

NA

Validity criteria fulfilled:

yes

Remarks:

mortality in controls < 10% and oxygen concentration > 3 mg/L

Conclusions:

The EC50 of the test material is > 667 mg/L
The EC50 of the substance is > 100 mg/L

Executive summary:

The substance (15% formulation) was tested in Daphnia magna (n=20, 4 replicates/concentration) according to OECD 202 in a static limit test. After 48 hours 35% immobility was seen at 667 mg/L (100 mg/L a.i.). The EC50 based on active ingredient is >100 mg/L.

Description of key information

The substance (15% formulation) was tested in Daphnia magna (n=20, 4 replicates/concentration) according to OECD 202 in a static limit test. After 48 hours 35% immobility was seen at 667 mg/L (100 mg/L a.i.). The EC50 based on active ingredient is >100 mg/L (IBACON 2007)

In a study performed according to OECD 202, daphnids were exposed to a formultion of the substance during 48 hours. Effects on mobility were found at 10 mg/L and above (based on test item). The EC50 is 10.4 mg/L (based on a.i.). The publication contains only limited information and it is not clear whether other components of the formulation may have influenced this result (Darsana 2015).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L