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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-11-27 to 1996-03-14
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted 4 April 1984
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 52.7, 79.0, 118.5, 177.8, 266.7, 400.0 mg/L; initial and final
- Sampling method: no data
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A homogeneous stock solution (400 mg/L) was prepared by stirring at 20°C for 48 h.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna STRAUS (Clone 5)
- Source: from laboratory’s own culture
- Age at study initiation (mean and range, SD): <24 h

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): same as test, Elendt M7 medium
- Type and amount of food: algae, Raphidocelis subcapitata
- Feeding frequency: no data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no
Test temperature:
19 - 21°C
pH:
7.49 - 7.88 (at 48 h)
Dissolved oxygen:
7.9 to 8.6 mg/L (at 48 h)
Nominal and measured concentrations:
nominal: 52.7, 79.0, 118.5, 177.8, 266.7, 400.0 mg/L
measured (initial): 53.4, 80.4, 122.1, 180.8, 268.4, 397.4 mg/L
measured (final): 49.6, 75.0, 114.0, 174.9, 261.8, 393.1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: closed flask glassware; to avoid volatilisation of the test substance, flasks were entirely filled with the test solutions and closed with butyl rubber caps covered with PTFE
- Material, size, headspace, fill volume: 120 mL nominal capacity
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (OECD202), Elendt M7

OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: no data
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Range finding study
- Test concentrations: 0.1 – 100 mg/L
- Results used to determine the conditions for the definitive study: no data
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
310 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: 95% c.i. 230-580 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
170 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: 95% c.i. 130-230 mg/L
Details on results:
- Other biological observations: none reported
- Mortality of control: 1/20 Daphnids (5%) were immobilised after 24 h; and 2/20 Daphnids (10%) were immobilised after 48 h
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50/LC50: 24 h-EC50 = 1.1 mg/L (performed 1996-02-07)
Reported statistics and error estimates:
probit analysis

Nominal concentration [mg/L]

Number of living Daphnids after 24 h

% immobilised after 24 h

Number of living Daphnids after 48 h

% immobilised after 48 h

0

19

5

18

10

52.7

19

5

18

10

79

20

0

20

0

118.5

17

15

13

35

177.8

15

25

7

65

266.7

13

35

5

75

400

7

65

0

100

Validity criteria fulfilled:
yes
Conclusions:
The 48 h EC50 of ETMA in Daphnia magna was 170 mg/L (95% c.i. 130-230 mg/L).
Executive summary:

The 48 h acute toxicity of ETMA to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test chemical at nominal concentration of 52.7, 79.0, 118.5, 177.8, 266.7, 400.0 mg/L for 48 h. Mortality/immobilization was observed daily.

 

The 24 h EC50 based on measured initial concentrations was 310 mg/L (95% c.i. 230 – 580 mg/L); the 48 h EC50 based on measured initial concentrations was 170 mg/L (95% c.i. 130-230 mg/L).

 

Results Synopsis

 

Test Organism Age: <24 h

Test Type: Static

 

48 h EC50:  170 mg/L                         95% c.i. 130-230 mg/L

Endpoint effected: immobilisation

Description of key information

48 h EC50=170 mg/L (95% c.i. 130-230 mg/L); OECD Guideline 202/ EU Method C.2; GLP

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
170 mg/L

Additional information

One short-term study in freshwater invertebrates is available for ETMA, which is considered reliable without restriction (Klimisch score 1).

The 48 h acute toxicity of ETMA to Daphnia magna was studied under static conditions according to OECD Guideline 202, adopted 4 April 1984 and EU Method C.2. Daphnids were exposed to control and test chemical at nominal concentration of 52.7, 79.0, 118.5, 177.8, 266.7, 400.0 mg/L for 48 h. Mortality/immobilisation was observed daily.

The 24 h EC50 based on measured initial concentrations was 310 mg/L (95% c.i. 230 – 580 mg/L); the 48 h EC50 based on measured initial concentrations was 170 mg/L (95% c.i. 130-230 mg/L).