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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012-08-27 to 2012-08-31
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 17 July 1992
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 6.25, 12.5, 25.0, 50.0, 100 mg/L
freshly prepared media: after 0 and 72 h, 24 hold test media: after 24 and 96 h
- Sample storage conditions before analysis: All original sampies were stored at 6 ± 2 °C until sample preparation, if necessary. Prepared samples were stored at room temperature until analysis.
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: Niedersächsischer Landesbetrieb für Wasserwirtschaft, Küsten- und Naturschutz, An der Scharlake 39, 31135 Hildesheim, Germany
- Length at study initiation (length definition, mean, range and SD): 2.4 cm
- Weight at study initiation (mean and range, SD): 0.243 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 12 d
- Type and amount of food: Sera Vipan; SERA GMBH; 4% of body weight per feeding day; fish were not fed 24 h before the test started
- Feeding frequency: 3 times/week
- Health during acclimation (any mortality observed): only fish with mortality <5% were used for the test
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
48 mg CaCO3/L
Test temperature:
23.5 ± 0.2 °C
pH:
7.47-7.84
Dissolved oxygen:
82-100% saturation
Nominal and measured concentrations:
nominal 6.25, 12.5, 25.0, 50.0, 100 mg/L;
measured concentrations in freshly prepared media were in the range of 94 - 98 % of the nominal values and 90 - 95 % in 24 h aged test media
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria of 14 L, filled with 10 L of dilution water
- Type: covered with a floating lid (aluminium foil) to reduce the loss of the test item via the surface
- Aeration: no
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: fish density in the tanks was 0.170 g fish per liter test solution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water of local origin, filtered on activated charcoal and aerated for at least 24 h to remove chlorine

OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: natural
- Light intensity: 2.59 to 3.03 µmol photons/m²/s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortality (considered dead if there was no visible movement): 24, 48, 72, 96 h
Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 10, 100 mg/L
- Results used to determine the conditions for the definitive study: 100% mortality at 100 mg/L
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
70.7 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
52.5 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
50.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
48.7 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
- 7/7 fish were dead after 24 h in the 100 mg/L group, 3/7 fish died in the 50 mg/L group, no mortality up to 25 mg/L
- Behavioural abnormalities: lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities up to 25 mg/L
- Mortality of control: 0/7 fish died
Results with reference substance (positive control):
n/a

Cumulative mortality

Nominal concentration [mg/L]

Cumulative mortality [%]

24 h

48 h

72 h

96 h

100

100

100

100

100

50

0

14

43

71

25

0

0

0

0

12.5

0

0

0

0

6.25

0

0

0

0

0 (control)

0

0

0

0

Validity criteria fulfilled:
yes
Conclusions:
The 96 h LC50 of ETMA in Danio rerio (zebrafish) was 48.7 mg a.i./L.
Executive summary:

In a 96-h acute toxicity study according to OECD Guideline 203, adopted 17 July 1992, Danio rerio (zebrafish) were exposed to ETMA at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg a.i./L under static renewal conditions.

The 96 h LC50 was 48.7 mg/L (based on nominal concentrations).

Sublethal effects like lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities were observed up to a concentration of 25 mg/L.

                                                                

Results Synopsis

Test organism size/age: Danio rerio, mean length 2.4 cm

Test Type: Static Renewal

LC50:  48.7 mg a.i./L

Endpoint(s) Effected: mortality

Description of key information

96 h LC50 = 48.7 mg/L; OECD guideline 203; GLP

Key value for chemical safety assessment

LC50 for freshwater fish:
48.7 mg/L

Additional information

One short-term study in freshwater fish is available for ETMA, which is considered reliable without restriction (Klimisch score 1).

In a 96-h acute toxicity study according to OECD Guideline 203, adopted 17 July 1992, Danio rerio (zebrafish) were exposed to ETMA at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg a.i./L under static renewal conditions.

Sublethal effects like lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities were observed up to a concentration of 25 mg/L. The 96 h LC50 was 48.7 mg/L (based on nominal concentrations).