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Ecotoxicological information

Endpoint summary

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Freshwater aquatic toxicity studies with ETMA were conducted in fish, invertebrates, and algal species.


Short-term toxicity to fish

In a 96-h acute toxicity study according to OECD Guideline 203, adopted 17 July 1992, Danio rerio (zebrafish) were exposed to ETMA at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg a.i./L under static renewal conditions.

The 96 h LC50 was 48.7 mg/L (based on nominal concentrations).


Short-term toxicity to aquatic invertebrates

The 48 h acute toxicity of ETMA to Daphnia magna was studied under static conditions according to OECD Guideline 202, adopted 4 April 1984 and EU Method C.2. Daphnids were exposed to control and test chemical at nominal concentration of 52.7, 79.0, 118.5, 177.8, 266.7, 400.0 mg/L for 48 h. Mortality/immobilisation was observed daily.

The 48 h EC50 based on measured initial concentrations was 170 mg/L (95% c.i. 130-230 mg/L).


Toxicity to aquatic algae

In a 72 hour toxicity study, the cultures of Pseudokirchneriella subcapitata CCAP 27814 were exposed to ETMA at nominal concentrations of 0 (control), 4.3, 9.4 20.7, 45.5, 100 and 200 mg/L under static conditions in accordance with OECD Guideline 201, adopted 07 June 1984, and EU test method C.3, 04 April 1992.

Microscopic observation showed that some algae exposed to the 2 highest concentrations appeared turgid in comparison to the control.

The EC10 and EC50 values based on growth rate were 22.0 mg/L and >184.2 mg/L, respectively. All effect levels are given based on initial measured concentrations of ETMA.


Toxicity to microorganisms

In a 3 h Respiration Inhibition Test according to OECD Guideline 209, adopted 22 July 2010, activated sludge obtained from a municipal treatment plant was exposed to ETMA at nominal concentrations of 10, 32, 100, 320 and 1000 mg/L under static conditions. In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item. The reference item produced the appropriate response.

The EC10 of ETMA based on respiration inhibition of activated sludge is 72.8 mg/L.


In conclusion, the lowest aquatic toxicity value for ETMA was the 96 h LC50 of 48.7 mg/L, based on the short-term toxicity study in Danio rerio.