Registration Dossier
Registration Dossier
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Reaction mass of Cobalt, 4-[4-[[4-[[[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1Hpyrazol-4-yl)azo]-4-hydroxyphenyl]sulfonyl]amino]phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulfonate 4-[4-[[4-[[[3-[[4,5-dihydro-3-methyl-5-oxo-1-(4-sulfophenyl)-1H-pyrazol-4-yl]azo]-4-hydroxyphenyl]sulfonyl]amino]phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1Hpyrazol-1-yl]benzenesulfonate sodium complexes and Cobaltate(3-), bis[4-[4-[[4-[[[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxyphenyl]sulfonyl]amino]phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1Hpyrazol-1-yl]benzenesulfonato(3-)]-, trisodium and Cobaltate(5-), bis[4-[4-[[4-[[[3-[[4,5-dihydro-3-methyl-5-oxo-1-(4-sulfophenyl)-1H-pyrazol-4-yl]azo]-4-hydroxyphenyl]sulfonyl]amino]phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1Hpyrazol-1-yl]benzenesulfonato(4-)]-, pentasodium
EC number: 916-898-0 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (48 h, Daphnia magna) = 38.33 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 38.33 mg/L
Additional information
The acute toxicity of the test item to Daphnia magna was evaluated in an experimental study according to the OECD Guideline 202 (2004) and the EU Method C.2 of the Regulation EC 440/2008. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the number of immobilised Daphnia were counted.
Three concentrations showed toxicity with between 5 and 90% immobilisation. One of the animals was immobilised in the blank control. Potassium dichromate K2Cr2O7 was used as positive control in a current reference study to assure that the test conditions are reliable. At the beginning and at the end of the test, the concentration of the test item in the test solutions was determined using a photometer method. The measured concentrations were between 103 % and 105 % of the nominal concentration at the start and after 48 hours between 95 % and 111 %. Therefore, the determination of the biological results was based on the nominal concentrations. The EC50 value of the test item when exposed to Daphnia magna for 48 hours was calculated to be 38.33 mg/l.
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