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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below:

1.Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material. The nominal concent ration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be 100 mg/L. Also the substance was found to be not rapidly degradable.Thus according to CLP Criteria for aquatic classification of the substance, it is concluded that test material exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category.

EC50 (48 hours) Experimental = 100 mg/L

2.Determination of the inhibition of the mobility of daphnids was carried out with the substance,according to OECD Guideline 202.

The test substance was tested at the concentrations 0, 6, 10, 17, 29, 50 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance,1-(3-chloropropyl)-1,3-dihydro-2H-benzimidazol-2-one, in Daphnia magna was determined to be 29.8 mg/L for immobilisation effects.

This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 29 to 100 mg/

L. Thus, comparing this value with the criteria of CLP regulation,

1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified

for short term toxicity for aquatic invertebrate .Hence,based on the data available for functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below.

1.The algal growth inhibition test was performed using as test material on Chlorella sp .

 Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .

 The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.

Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.

2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.

The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified

for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.

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Additional information

Short-term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below:

1.Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material. The nominal concent ration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be 100 mg/L. Also the substance was found to be not rapidly degradable.Thus according to CLP Criteria for aquatic classification of the substance, it is concluded that test material exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category.

EC50 (48 hours) Experimental = 100 mg/L

2.Determination of the inhibition of the mobility of daphnids was carried out with the substance,according to OECD Guideline 202.

The test substance was tested at the concentrations 0, 6, 10, 17, 29, 50 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance,1-(3-chloropropyl)-1,3-dihydro-2H-benzimidazol-2-one, in Daphnia magna was determined to be 29.8 mg/L for immobilisation effects.

This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 29 to 100 mg/

L. Thus, comparing this value with the criteria of CLP regulation,

1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified

for short term toxicity for aquatic invertebrate .Hence,based on the data available for functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below.

1.The algal growth inhibition test was performed using as test material on Chlorella sp .

 Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .

 The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.

Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.

2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.

The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified

for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.