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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to
Guideline:
other: Japanese Industrial Standard JIS K0102
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
yes
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: carp, prelarvae, 1 week old
- Source: Fish farm in Tokyo
- Length at study initiation: 9 mm
- Weight at study initiation: 4.4 mg

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Hardness:
25 mg/L as CaCO3
Test temperature:
22 °C
pH:
7.0
Dissolved oxygen:
7.2 mg/L
Nominal and measured concentrations:
not reported
Details on test conditions:
TEST SYSTEM
- Test vessel: 800 mL beaker
- Renewal rate of test solution: the water was not changed during the experiment
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: softened water was used.

EFFECT PARAMETERS MEASURED: mortality
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

6h-LC50: 190 mg/L

12h-LC50: 180 mg/L
24h-LC50: 180 mg/L

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
02 Feb - 06 Feb 1998
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. certificate)
Remarks:
Freie und Hansestadt Hamburg, Behörde für Arbeit, Gesundheit und Soziales
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: golden orfe
- Source: Fish farm eggers, Martensweg 18, 24594 Hohenwestedt, Germany
- Length at study initiation: 5.5-6.3 cm
- Feeding during test: not fed for 24 hours before the start of the test


ACCLIMATION
- Acclimation period: 12 day acclimation in 70 L tank with aeration
- Acclimation conditions: for 7 days conditions similar to test conditions; 19.8-20.8 °C, 75.8-98.4% oxygen saturation, semi-static, pH: 7.33-7.87, 16 hours photoperiod, aerated
- Type and amount of food: flocculent food Idus-Fit (Interquell, 86517 Wehringen, Germany)
- Health during acclimation: 0% mortality in 7-day acclimation period

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
none
Hardness:
250 mg CaCO3/L
Test temperature:
20.0 - 21.1 °C
pH:
control: 7.47-7.95
100 mg/L: 7.19-7.95
Dissolved oxygen:
control: 77.5 - 98.8% (air saturated value)
100 mg/L: 51.5 - 98.7 % (air saturated value)
Nominal and measured concentrations:
nominal: 0 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 L tank
- Material, size, headspace, fill volume: 10 L
- Aeration: via pasteur pipettes (2 to 3 air bubbles per second)
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10 per tank
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Ca/Mg ratio: 4:1
- Na:K 10:1


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark


EFFECT PARAMETERS MEASURED: mortality, behaviour and appearance after 2-4 h, 24, 28, 72 and 96 h


Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality: Two out of ten fish died at the test concentration 100 mg/L (one after 72 and one after 96-hour exposure period).
- Behavioural abnormalities: None, one fish was found in angular position
- Observations on body length and weight: no difference in weight and length between control and 100 mg/L
Results with reference substance (positive control):
not reported
Reported statistics and error estimates:
No LC50 was calculated since a limit test was performed.
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Remarks:
Summary of available data used for the endpoint assessment of the target substance
Adequacy of study:
key study
Justification for type of information:
Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
Reason / purpose:
read-across source
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
Source, key, 142-87-0, 1976

Description of key information

48hr LC50 (Cyprinus carpio pre-larvae, mortality) = 13 mg/L (nominal concentration, Japanese Industrial Standard JIS K0102)

Key value for chemical safety assessment

LC50 for freshwater fish:
13 mg/L

Additional information

Since no reliable acute toxicity test with fish is available for Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to other structurally related substances was conducted. As the toxicity of alkyl sulfates is dependent on the carbon chain length, it is expected that the toxic effects are comparable for the similar homologues.

In the key, non-GLP study performed with C10AS Na (CAS 142-87-0) according to Japanese Industrial Standard JIS K0102, 1-week old carp pre-larvae (Cyprinus carpio) were exposed to C10AS Na for 48 hours under static conditions (Kikuchi et al. 1976a). Based on pre-larvae mortality, the LC50 value was 13 mg/L (nominal concentrations in agreement with the measured ones). Since the obtained value was the most critical, it is considered as a reliable short-term LC50 value for fish.

One GLP compliant study investigating effects of C12-isoAS Na (CAS 94200-74-5) is available (Sasol, 1998). The test was performed with golden orfe fish (Leuciscus idus) according to OECD guideline 203. Mortality of fish caused by the test substance was determined after 24, 48, 72 and 96 hours of exposure to the nominal test concentration of 100 mg/L in a limit test. No analytical measurement of test concentration is available. The results showed that the 96-hour LC50 value was above 100 mg/L (nominal concentration).

Due to structural and property similarities with the tested substances, it can be concluded that the above results apply also to Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt.