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REACH

Ammonium iodide

EC number: 234-717-7 | CAS number: 12027-06-4

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Ammonium iodide

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Ammonium iodide
- IUPAC name: Ammonium iodide
- Molecular formula: H4IN
- Molecular weight: 144.939 g/mole
- Smiles : [NH4+].[IH-]
- Inchl: 1S/HI.H3N/h1H;1H3
- Substance type: Inorganic
- Physical state: Solid crystalline powder (colorless to white)
-Batch No.: A1274

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 200 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) for achieving test concentrations of 100mg/L,respectively

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source:Anytime Pet,Jaripatka, Nagpur
- Length at study initiation : average 1.29 cm
- Weight at study initiation : 0.049g (average)

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions :The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along
with proper feed and aeration.
- Type and amount of food during acclimation: Standard brand feed
- Feeding frequency during acclimation: 1 day before and during exposure

- Health/mortality: no mortality observed

FEEDING DURING TEST
- Food type: Standard brand feed
- Frequency: once daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24, 48, 72 and 96 hours of the start of the experiment

Hardness:

153.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.1

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

100mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: plastic aquaria
- fill volume: 2 liters
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortality observed

Sublethal observations / clinical signs:

1. Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
Nominal Concentration (mg/l)	Number of fish		3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

24h

48h

72h

96h

Control

NS4

100

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
Nominal Concentration (mg/l)	Number of fish		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.2	7.2	7.3	7.3	7.3	7.3	7.4	7.4
100	4	4	7.6	7.6	7.7	7.7	7.7	7.7	7.8	7.8

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
Nominal Concentration (mg/l)	Number of fish		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	6.9	6.9	6.8	6.8	6.8	6.7	6.7	6.7
100	4	4	6.8	6.8	6.7	6.7	6.7	6.7	6.6	6.6

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
Nominal Concentration (mg/l)	Number of fish		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.2	23.2	23.3	23.3	23.3	23.3	23.4	23.4
100	4	4	23.3	23.3	23.2	23.3	23.4	23.4	23.4	23.4

Validity criteria fulfilled:

not specified

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 12027-06-4 on Zebra fish(Danio rerio)was found to be >100 mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Ammonium iodide .The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for Ammonium iodide on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L. Thus, on the basis

of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance,Ammonium iodide (CAS No. 12027 -06 -4) does not exhibit short term toxicity to fish.

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

Description of key information

Short-term toxicity to fish:

Short-term toxicity to fish study was conducted by Sustainability Support Services (Europe) AB as summarized below:

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 100 mg/L

Additional information

Short-term toxicity to fish:

Short-term toxicity to fish study was conducted by Sustainability Support Services (Europe) AB as summarized below:

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

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