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REACH

(S)-2-methyl-5-(1-methylvinyl)cyclohex-2-en-1-one

EC number: 218-827-2 | CAS number: 2244-16-8

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The experimental phase of the main study started at 23-01-2018 and lasted for a period of 96 hrs.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one- Common name: d-Carvone- Molecular formula: C10H14O- Molecular weight: 150.22 g/mol- Smiles notation: C1[C@H](CC=C(C1=O)C)C(C)=C- InChl: 1S/C10H14O/c1-7(2)9-5-4-8(3)10(11)6-9/h4,9H,1,5-6H2,2-3H3/t9-/m0/s1- Substance type: Organic- Physical state: colorless liquid-Purity:99.52%-Solubility in Water:1879.65 mg/L

Analytical monitoring:

yes

Details on sampling:

-Storage Conditions:Ambient Temp (23 to 27 °C)

Vehicle:

yes

Remarks:

potable water

Details on test solutions:

No data available

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name: Zebra fish- Source:Anytime Pet,Jaripatka, Nagpur- Length at study initiation :average 1.78 cm- Weight at study initiation :0.080g (average)ACCLIMATION- Acclimation period: 7 days- Type and amount of food during acclimation:Standard brand feed- Feeding frequency during acclimation:Daily once

Test type:

static

Water media type:

freshwater

Limit test:

Total exposure duration:

96 h

Post exposure observation period:

No mortailty was observed till 48 hrs

Hardness:

150.5

Test temperature:

23 °C

pH:

7.2

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L

Details on test conditions:

TEST SYSTEM - Test vessel: plastic aquaria - fill volume: 5 liter - No. of organisms per vessel:8-Areation:Aeration in test vessels was provided 1 day before the start of experiment

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
Nominal Concentration (mg/l)	Number of fish		3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
6.25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
12.5	4	4	-	-	-	-	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	2	2	2	2	2	2	2	2
100	4	4	2	2	4	4	4	4	4	4	4	4	4	4

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

24h

48h

72h

96h

Control

NS4

6.25

NS4

12.5

NS4

100

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
Nominal Concentration (mg/l)	Number of fish		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.5	7.5	7.7	7.7	7.9	7.9	8.0	8.0
6.25	4	4	7.7	7.7	7.8	7.8	8.0	8.0	8.1	8.1
12.5	4	4	7.7	7.7	7.8	7.8	8.0	8.0	8.3	8.3
25	4	4	7.7	7.7	7.9	7.9	8.0	8.0	8.3	8.3
50	4	4	7.8	7.8	7.9	7.9	8.1	8.1	8.3	8.3
100	4	4	-	-	-	-	-	-	-	-

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
Nominal Concentration (mg/l)	Number of fish		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6
6.25	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
12.5	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
25	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
50	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
100	4	4	-	-	-	-	-	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.2244-16-8 on Zebra fish(Danio rerio)was found to be 50 mg/L.

Executive summary:

A 96 h study was conducted for CAS No. 2244-16-8 on Zebra fish(Danio rerio), at a test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 50 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L

LC50 (96 hours) Experimental = 50 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L.

Based on the above effect concentration (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one can be classified as aquatic chronic 3 .

Eventhough the experimental result is considered (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one is toxic for fish but

(5S)-2-methyl-5- (prop-1-en-2-yl)cyclohex-2-en-1-one is readily biodegradable . Hence it can be considered Not classified as per CLP classification.

Description of key information

LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L

LC50 (96 hours) Experimental = 50 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L.

Based on the above effect concentration (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one can be classified as aquatic chronic 3 .

Eventhough the experimental result is considered (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one is toxic for fish but

(5S)-2-methyl-5- (prop-1-en-2-yl)cyclohex-2-en-1-one is readily biodegradable . Hence it can be considered Not classified as per CLP classification.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 50 mg/L

Additional information

The experimental study was conducted by NS Support Services to evaluate short teerm toxicity to fish of test chemical (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one

LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L

LC50 (96 hours) Experimental = 50 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L.

Based on the above effect concentration (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one can be classified as aquatic chronic 3 as per CLP classification.

Eventhough the experimental result is considered (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one is toxic for fish but

(5S)-2-methyl-5- (prop-1-en-2-yl)cyclohex-2-en-1-one is readily biodegradable . Hence it can be considered Not classified as per CLP classification.

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