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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental results of read across chemicals
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals.
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
WoE report is prepared based on short term toxicity to aquatic invertebrates studies
1 and 2
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): Castor oil, sulfated
- Molecular formula: C57H101O18S3Na3
- Molecular weight: 1239.5749 g/mol
- Smiles notation: CCCCCCC(OS(=O)(=O)[O-])C/C=C\CCCCCCCC(=O)OCC(OC(=O)CCCCCCC/C=C\CC(OS(=O)(=O)[O-])CCCCCC)COC(=O)CCCCCCC/C=C\CC(OS(=O)(=O)[O-])CCCCCC.[Na+].[Na+].[Na+]
- InChI: 1S/C57H104O18S3.3Na/c1-4-7-10-31-40-51(73-76(61,62)63)43-34-25-19-13-16-22-28-37-46-55(58)70-49-54(72-57(60)48-39-30-24-18-15-21-27-36-45-53(75-78(67,68)69)42-33-12-9-6-3)50-71-56(59)47-38-29-23-17-14-20-26-35-44-52(74-77(64,65)66)41-32-11-8-5-2;;;/h25-27,34-36,51-54H,4-24,28-33,37-50H2,1-3H3,(H,61,62,63)(H,64,65,66)(H,67,68,69);;;/q;3*+1/p-3/b34-25-,35-26-,36-27-;;;
- Substance type: Organic
- Physical Appearance pale yellow liquid
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
1. The stock solution 10 g/l was prepared by dissolving white powder in acetone. For a final concentration of 100 mg/l in the test solution the final stock solution was mixed in reconstituent water. It was possible to test at higher substance concentration due to the formation of precipitate.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
± 1 hr
Test temperature:
1. 20±1°C
2. 20.4 to 20.7° C
pH:
1. Test: 7.9 (No changed during test)
Control: 8 (change to 7.9 during test)
Control + acetone: 8 (change to 7.9 during test)

2. 7.7 to 7.9
Dissolved oxygen:
higher than 7.9 mg/L at the end of test
Nominal and measured concentrations:
0, 10 mg/l
Details on test conditions:
1. TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
1. Potassium dichromate (K2Cr2O7)
Key result
Duration:
48 h
Dose descriptor:
other: IC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 1: 4 % inhibition were observed
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Other details not known
Results with reference substance (positive control):
1. - Results with reference substance valid
- EC50: 0.73 mg/L (24 hours)
Validity criteria fulfilled:
not specified
Conclusions:
1. As the chemical was not highly soluble in water thus test conducted at 10 mg/l. After the exposure of 48 hrs, only 4 % immobility were observed in Daphnia magna. Thus on that criteria chemical was not consider as toxic. Based on the study chemical was may be consider as nontoxic and notclassified as per the CLP criteria.
2. Based on the immobility of daphnia magna by providing the exposure of test chemical for 48 hrs, the EC50 was determine to be > 5 mg/l.
On the basis of the experimental studies and all the data summarized for Castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates of the test chemical Castor oil, sulfated. The studies are as mentioned below: 

Aim of this study was to assess the short term toxicity of to test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline 202 in a static system for the total exposure period of 48 hrs. The stock solution 10 g/l was prepared by dissolving white powder in acetone. For a final concentration of 100 mg/l in the test solution the final stock solution was mixed in reconstitute water.  It was possible to test at higher substance concentration due to the formation of precipitate. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. As the chemical was not highly soluble in water thus test conducted at 10 mg/l. After the exposure of 48 hrs, only 4 % immobility were observed in Daphnia magna. Thus on that criteria chemical was not consider as toxic. Based on the study chemical was may be consider as nontoxic and not classified as per the CLP criteria.

 

Similarly another study was conducted to evaluate the effect of test chemical on the mobility of daphnia magna by providing the exposure period of 48 hrs. Test conducted in accordance with the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Study performed under the static system. Based on the immobility of daphnia magna by providing the exposure of test chemical for 48 hrs, the EC50 was determine to be > 5 mg/l. As the chemical was readily degradable in water thus before causing any toxicity on the daphnia and other aquatic invertebrates it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

On the basis of the experimental studies and all the data summarized for Castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

1. As the chemical was not highly soluble in water thus test conducted at 10 mg/l. After the exposure of 48 hrs, only 4 % immobility were observed in Daphnia magna. Thus on that criteria chemical was not consider as toxic. Based on the study chemical was may be consider as nontoxic and notclassified as per the CLP criteria.

2. Based on the immobility of daphnia magna by providing the exposure of test chemical for 48 hrs, the EC50 was determine to be > 5 mg/l.

On the basis of the experimental studies and all the data summarized for Castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Additional information

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates of the test chemical Castor oil, sulfated. The studies are as mentioned below: 

Study from experimental study report 2017, Aim of this study was to assess the short term toxicity of to test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline 202 in a static system for the total exposure period of 48 hrs. The stock solution 10 g/l was prepared by dissolving white powder in acetone. For a final concentration of 100 mg/l in the test solution the final stock solution was mixed in reconstitute water.  It was possible to test at higher substance concentration due to the formation of precipitate. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. As the chemical was not highly soluble in water thus test conducted at 10 mg/l. After the exposure of 48 hrs, only 4 % immobility were observed in Daphnia magna. Thus on that criteria chemical was not consider as toxic. Based on the study chemical was may be consider as nontoxic and not classified as per the CLP criteria.

 

Similarly another study from authoritative database was conducted to evaluate the effect of test chemical on the mobility of daphnia magna by providing the exposure period of 48 hrs. Test conducted in accordance with the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Study performed under the static system. Based on the immobility of daphnia magna by providing the exposure of test chemical for 48 hrs, the EC50 was determine to be > 5 mg/l. As the chemical was readily degradable in water thus before causing any toxicity on the daphnia and other aquatic invertebrates it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

On the basis of the experimental studies and all the data summarized for Castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.