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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the OECD guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound Castor oil, sulfated on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): Castor oil, sulfated
- Molecular formula: C57H101O18S3Na3
- Molecular weight: 1239.5749 g/mol
- Smiles notation: CCCCCCC(OS(=O)(=O)[O-])C/C=C\CCCCCCCC(=O)OCC(OC(=O)CCCCCCC/C=C\CC(OS(=O)(=O)[O-])CCCCCC)COC(=O)CCCCCCC/C=C\CC(OS(=O)(=O)[O-])CCCCCC.[Na+].[Na+].[Na+]
- InChI: 1S/C57H104O18S3.3Na/c1-4-7-10-31-40-51(73-76(61,62)63)43-34-25-19-13-16-22-28-37-46-55(58)70-49-54(72-57(60)48-39-30-24-18-15-21-27-36-45-53(75-78(67,68)69)42-33-12-9-6-3)50-71-56(59)47-38-29-23-17-14-20-26-35-44-52(74-77(64,65)66)41-32-11-8-5-2;;;/h25-27,34-36,51-54H,4-24,28-33,37-50H2,1-3H3,(H,61,62,63)(H,64,65,66)(H,67,68,69);;;/q;3*+1/p-3/b34-25-,35-26-,36-27-;;;
- Substance type: Organic
- Batch No. G-7969
- Physical Appearance pale yellow liquid
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
Method: The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish(Danio rerio)
- Source: Anytime Pet, Jariphataka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.47 cm
- Weight at study initiation (mean and range, SD): 0.044 g (average)


ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): Same as test
- Feeding frequency during acclimation: Daily once
- Health during acclimation (any mortality observed): No mortality were observed

Other conditions during acclimations:
Photoperiod 16 hours light and 8 hours dark
Temperature range 23.6 °C
pH range 7.5
DO range 7.0 mg/l
Feeding Daily once
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Post exposure observation period:
24, 48, 72, 96 hrs
Hardness:
150.5 (mg of CaCO3)
Test temperature:
23 °C
pH:
7.2
Dissolved oxygen:
7 mg/l
Nominal and measured concentrations:
6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water with 3 liter of headspace.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 2


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light and 8 hours dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations of (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.


Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: No effect were observed at 12 mg/l
Key result
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Toxic

Fig: Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

6.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

12.5

4

4

-

-

-

-

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

2

2

2

2

50

4

4

-

-

-

-

-

-

4

4

4

4

4

4

100

4

4

-

-

4

4

4

4

4

4

4

4

4

4

Fig: Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

4

4

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

6.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

12.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

50

4

4

NS4

NS4

NS4

NS4

NS4

NS4

-

-

-

-

-

-

100

4

4

NS4

NS4

-

-

-

-

-

-

-

-

-

-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation


 

 

 

 

 

Fig: pH and oxygen content:

 

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

7.2

7.2

7.3

7.3

7.4

7.4

7.7

7.7

6.25

4

4

7.7

7.7

7.8

7.8

7.9

7.9

8.1

8.1

12.5

4

4

7.7

7.7

7.9

7.9

8.0

8.0

8.2

8.2

25

4

4

7.8

7.8

7.9

7.9

8.0

8.0

8.2

8.2

50

4

4

7.8

7.8

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.9

6.9

6.8

6.8

6.7

6.7

6.6

6.6

6.25

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.4

6.4

12.5

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.4

6.4

25

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.4

6.4

50

4

4

6.9

6.9

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.2

23.2

22.9

22.9

23.0

23.0

23.4

23.4

6.25

4

4

23.2

23.2

22.9

22.9

23.0

23.0

23.4

23.4

12.5

4

4

23.2

23.2

22.9

22.9

23.0

23.0

23.4

23.4

25

4

4

23.2

23.2

22.9

22.9

23.0

23.0

23.4

23.4

50

4

4

23.2

23.2

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

 

 

 

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Castor oil, sulfated on Zebra fish (Danio rerio) was determined to be 25 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Executive summary:

This study was designed to assess the toxic effects of the test compound Castor oil, sulfated on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.

Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.  

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Castor oil, sulfated on Zebra fish (Danio rerio) was determined to be 25 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. As the chemical was readily degradable in water thus before causing any toxicity on the fishes it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Castor oil, sulfated on Zebra fish (Danio rerio) was determined to be 25 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. As the chemical was readily degradable in water thus before causing any toxicity on the fishes it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
25 mg/L

Additional information

This study was designed to assess the toxic effects of the test compound Castor oil, sulfated on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Castor oil, sulfated on Zebra fish (Danio rerio) was determined to be 25 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. As the chemical was readily degradable in water thus before causing any toxicity on the fishes it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.