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EC number: 232-884-0 | CAS number: 9032-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 August 1992-30 November 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Active enzyme protein of Lipase, triacylglycerol (EC no. 232-619-9, CAS no. 9001-62-1, EC name: Lipase, triacylglycerol, Enzyme Class No.: 3.1.1.3)
- Molecular formula:
- Not applicable, see remarks
- IUPAC Name:
- Active enzyme protein of Lipase, triacylglycerol (EC no. 232-619-9, CAS no. 9001-62-1, EC name: Lipase, triacylglycerol, Enzyme Class No.: 3.1.1.3)
- Reference substance name:
- Protein as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available
- IUPAC Name:
- Protein as a constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Carbohydrates constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Carbohydrates constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Lipids as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Lipids as a constituent of enzyme deriving from the fermentation or extraction process
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Lot/batch No.: PPW 3983
- Expiration date of the lot/batch: 2 September 1994
- Stability under test conditions: The test enzyme and dilutions in water (10% and 100%) are stable for at least 24 hours at room temperature
- Storage condition of test material: In the dark at 4⁰C
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Constituent 5
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in sterile nutrient medium to provide an initial stock solution of 320 mg/L. This stock solution was further diluted with sterile nutrient medium to produce a series of solutions exactly twice the concentration of the intended exposure levels. 150 mL of algal pre-culture was mixed with 150 mL of each of these solutions to give the final test series.
- Controls: Medium
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: CCAP 276/20
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa c/o Freshwater Biological Association, Cumbria, UK
- Method of cultivation: Sterile nutrient medium was inoculated from a master culture and incubated under continuous illumination (appr. 7000 lux) and stirring (orbital shaker) at 24 +/- 1°C to give an algal suspension in log phase growth characterised by an absorbanse of 0.380 (@665nm).
The suspension was diluted using sterile nutrient medium to an absorbance of 0.036 prior to use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23-25°C
- pH:
- 7.5 - 10.3
- Nominal and measured concentrations:
- nominal: 0, 10, 20, 40, 80 and 160 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flask
- Type (delete if not applicable): Flasks were loosely stoppered.
- Material, size, fill volume: glass, 250 mL, 100 mL
- Initial cells density: 7.9 x 10^4 cells/mL
- Control end cells density: 2.3 x 10^6 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: Sterile nutrient medium as recommended in OECD Guideline No. 201
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse-osmosis purified water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: approximately 7000 lux provided by 7 x 30 W "warm white" 1 metre fluorescent tubes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Samples were taken at 0, 24, 48 and 72 hours and the cell densities measured at 665 nm using 4 cm path cuvettes in a Cecil 373 Series 2 Spectrophotometer. The cell densities of control cultures at initiation and at termination were determinated by direct counting in a haemacytometer. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 40 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 7.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- aep (active enzyme protein)
- Basis for effect:
- other: growth rate and biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- aep
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 99 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- aep
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): At the highesr exposure level of 160 mg/L cells were observed to be clumped. No abnormalities were obsereved at all other exposure concetrations inclduing controls. - Reported statistics and error estimates:
- In order to estimate the concentration at which 50% inhibition occurs, a logistic model (Volund, A. 1978. Biometrics 34) was fitted to the percentage inhibition values based on the 'area under the curve' (AUC) at 72 hours and on the growth rate between 24 and 72 hours.
The 50% points were estimated by interpolation of the fitted curves and 95% confidence limits were produced by reference to Student's t distribution, using a pooled estimate of the error variance about the model (Draper and Smith, 1966. Applied Regression Ananlyisi (first ed.) John Wiley and Sons, Inc. New York).
A 'no observed effect level' was obtained using Williams test (Williams, D.A., 1971. Biometrics 27. Williams, D.A.1972. Biometrics 28). Bartletts test (Bartlett, M.S. 1973. J.Royal Stat.Soc.Suppl.4) with a 1% significance level was used for homogeneity of variance.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Lipolase is inhibitory to the growth of Scenedesmus subspicatus (new name: Desmodesmus subspicatus) at concentration in excess of 40 mg/L or 7.4 mg active enzyme protein (aep)/L. The 72h EbC50 is 97 mg/L or 17.6 mg aep/L and the ErC50 is 99 mg /L or 18 mg aep/L.
- Executive summary:
The effect of Lipolase on the growth of the unicellular green alga Scenedesmus subspicatus (new name
Desmodesmus subspicatus) was assessed in accordance with EEC Methods for the Determination of Ecotoxicity, EEC Directive 67/548 Annex VIII Part C (87/302/EEC) and OECD test guideline 201, and in compliance with GLP.
Algal cultures exposed to five test concentrations of Lipolase plus one untreated control were incubated on an orbital shaker under continuous illumination at 24 ± 1°C for 72 hours. Growth was monitored daily by measuring the absorbance of each culture at 665 nm.
The following values were derived from the data:
Nominal Lipolase concentration
(mg/L)
Nominal Lipolase concentration
(mg aep/L)
Area under the growth curve
EbC50 (72 h)
97 17.6 Average specific growth rate
ErC50 (0 - 72 h)
99 18
“No observed effect concentration”
40 7.4
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