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EC number: 609-221-2 | CAS number: 3625-46-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
- Principles of method if other than guideline:
- Ames II Assay according to Gee, P. et al. (1998) and on the "Users Manual" prepared by XENOMETRIX, Inc., Colorado, USA
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,7,11-trimethyldodec-1-en-3-ol
- EC Number:
- 609-221-2
- Cas Number:
- 3625-46-5
- Molecular formula:
- C15H30O
- IUPAC Name:
- 3,7,11-trimethyldodec-1-en-3-ol
- Test material form:
- liquid
- Details on test material:
- Tetrahydronerolidol (THNER)
CAS No. 3625-46-5
Batch No.: Anlage 067, 13 Jun 2001
Purity: 96 %
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: Anlage 067, 13.06.01
- Date of manufacture: June 2001
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: The stability of the test substance throughout the study has not been verified by reanalysis. The analytical investigations were carried out in the Analytical Departmetn, BASF AG (characterization)
- Stability of the test substance in the vehicle: The stability of the test substance in the vehicle DMSO and in water has not been determined analytically. Detailed information may be requested from the sponsor BASF AG.
Method
- Target gene:
- his
Species / strainopen allclose all
- Species / strain / cell type:
- other: Salmonella typhimurium TA Mix (Mixed strains TA 7001 - TA 7006) Liquid Fluctuation Test - Microtiter Version
- Species / strain / cell type:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9-mix
- Test concentrations with justification for top dose:
- 4, 20, 100, 500, 2500 and 5000 µg/ml
- Vehicle / solvent:
- - Vehicle used: DMSO
- Justification for choice of vehicle: Due to the limited solubility of the test substance in water, DMSO was selected as the vehicle, which had been demonstrated to be suitable in bacterial reverse mutation tests and for which historical control data are available
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2-AA); 5 µg/ml
- Remarks:
- with metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Mixture of 2-nitrofluorene (2-NF; 0.25 µg/ml) and 4-nitroquinoline-N-oxide (4-NQO; 0.0625 µg/ml)
- Remarks:
- without metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium (liquid fluctuation test with and without S-9 mix)
DURATION
- Exposure duration: 90 min, 37°C
- Selection time (if incubation with a selection agent): ca. 48, 37°C
SELECTION AGENT (mutation assays): histidine-deficient Ames II Reversion Indicator medium (Xenometrix)
NUMBER OF REPLICATIONS: Triplicate plates per dose, control chemical or vehicle
DETERMINATION OF CYTOTOXICITY
- Method: decrease in the number of positive wells, clearing or diminution of the background lawn (= reduced his' background growth) leading from turbid to non-turbid purple wells
OTHER: Acceptance criteria
Generally, the experiment is considered valid if the following criteria are met:
- the number of revertant wells in the negative controls was within the normal range of the historical control data for each tester strain
- the sterility controls revealed no indication of bacterial contamination
- the positive control articles both with and without S-9 mix induced a significant
increase in the number of positive wells within the range of the historical control data
- Evaluation criteria:
- EVALUATION CRITERA
- The test chemical is considered positive in this assay if the following criteria are met:
a dose-related increase in the number of positive wells by a factor of about 2 (calculated primarily on the basis of baseline data) in at least one tester strain either without S-9 mix or after adding a metabolizing system.
- A test substance is generally considered non-mutagenic in this test if:
the number of revertant wells for all tester strains were within the historical negative control range under all experimental conditions
ACCEPTANCE CRITERIA
Generally, the experiment is considered valid if the following criteria are met :
• The number of revertant wells in the negative controls was within the normal range of the historical control data for each tester strain
• The sterility controls revealed no indication of bacterial contamination .
• The positive control articles both with and without S-9 mix induced a significant increase in the number of positive wells within the range of the historical control data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- other: Salmonella typhimurium TA Mix (Mixed strains TA 7001 - TA 7006) Liquid Fluctuation Test - Microtiter Version
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No test substance precipitation was found
Any other information on results incl. tables
Result:
Dose µg/plate | metabolic activation | TA98 | TA Mix (Mixed strains TA 7001 - TA 7006) | ||||
Plate 1 | Plate 2 | Plate 3 | Plate 1 | Plate 2 | Plate 3 | ||
DMSO | - | 2 | 3 | 3 | 1 | 1 | 1 |
4 | - | 3 | 1 | 0 | 0 | 1 | 1 |
20 | - | 1 | 0 | 0 | 0 | 0 | 0 |
100 | - | 0 | 1 | 2 | 1 | 1 | 0 |
500 | - | 1 | 3 | 0 | 0 | 0 | 2 |
2500 | - | 2 | 2 | 1 | 1 | 0 | 1 |
5000 | - | 5 | 0 | 1 | 0 | 1 | 1 |
4-NQO + 2-NF | - | 30 | 20 | 22 | 28 | 23 | 25 |
DMSO | + | 3 | 2 | 5 | 0 | 1 | 2 |
4 | + | 6 | 1 | 1 | 1 | 2 | 0 |
20 | + | 0 | 3 | 1 | 2 | 1 | 1 |
100 | + | 1 | 0 | 5 | 0 | 0 | 1 |
500 | + | 3 | 2 | 1 | 0 | 0 | 0 |
2500 | + | 1 | 1 | 3 | 1 | 0 | 0 |
5000 | + | 3 | 4 | 3 | 1 | 0 | 1 |
2-AA | + | 48 | 48 | 48 | 29 | 27 | 24 |
Applicant's summary and conclusion
- Conclusions:
- Thus, under the experimental conditions chosen here, it is concluded that the test substance is not a mutagenic agent in the Ames II Assay (Salmonella typhimurium reverse mutation assay) .
- Executive summary:
The test substance was tested for its mutagenic potential based on the ability to induce point mutations in selected loci of several strains of Salmonella typhimurium in a modified version of the traditional Ames test, i .e. the Ames II Assay (microtiter version) .
STRAINS: TA 98, TA Mix (Mixed strains TA 7001 - TA 7006)
DOSE RANGE: 4 µg - 5,000 µg/ml
TEST CONDITIONS: Liquid fluctuation test both with and without metabolic activation (Aroclor-induced rat liver S-9 mix)
SOLUBILITY: No precipitation of the test substance was found
TOXICITY: No bacteriotoxic effect was observed
MUTAGENICITY: An increase in the number of positive wells (his+ revertants) was not observed either without S-9 mix or after the addition of a metabolizing system
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