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EC number: 223-804-5 | CAS number: 4079-68-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Pseudomonas growth inhibition test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Golpanol DEP
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- CULTURING CONDITIONS
- Strain: DSM 50026
- Temperature: 23 - 25 °C
- Shaking: yes; 150 rpm
- Vessel: 300 mL Erlenmeyer, containing 100 mL culture medium
- Growth period: 6 - 8 h
- Pretreatment: No pretreatment - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Test temperature:
- 20 °C
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 39.531, 78.125, 156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/L (based on a range-finding study).
- Details on test conditions:
- TEST APPROACH
All test vessels contain 0.5 mL nutrient solution concentrate 1, 0.5 mL nutrient solution concentrate 2, 1 mL bacterial suspension in NaCl solution set to 10 TE/F and 8 mL test substance solution (concentration f = 1.25).
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST PARAMETERS MEASURED: optical density
Bacterial growth (inhibition) was determined optically by means of spectrophotometry at a wavelength of 436 nm and compared to a blank control.
RANGE-FINDING STUDY
- Nominal concentrations: 0 (control), 15.625, 31.25, 62.5, 125, 250, 500, 100 and 2000 mg/L.
- Results used to determine the conditions for the definitive study: Up to 250 mg/L, no inhibition was observed. From 500 to 2000 mg/L, inhibition increased in a dose-dependent manner. The EC50 and EC10 values were determined to be 1149.5 and 4664 mg/L, respectively. - Reference substance (positive control):
- no
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 628 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 769 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- Up to and including the dose group of 625 mg/L, no inhibition was observed. From 1250 mg/L to 10000 mg/L, the measured density decreased in a dose-dependent manner from 65.81% to 0.24 % relative to the control, respectively. An overview of the results is provided in 'Any other information on results incl. tables'.
- Validity criteria fulfilled:
- not specified
Reference
Table: Optical density of the P. putida cell suspension compared to control measured at 436 nm.
Concentration in mg/L |
Inoculated |
Uninoculated |
Difference |
% of control |
Control |
0.418 |
--- |
--- |
--- |
39.531 |
0.426 |
0.002 |
0.424 |
101.62 |
78.125 |
0.433 |
0.002 |
0.431 |
103.11 |
156.25 |
0.438 |
0.001 |
0.437 |
104.73 |
312.5 |
0.446 |
0.001 |
0.445 |
106.59 |
625 |
0.407 |
0.001 |
0.406 |
97.25 |
1250 |
0.276 |
0.001 |
0.275 |
65.81 |
2500 |
0.059 |
0.002 |
0.057 |
13.53 |
5000 |
0.002 |
0.000 |
0.002 |
0.48 |
7500 |
0.001 |
0.000 |
0.001 |
0.24 |
10000 |
0.001 |
0.000 |
0.001 |
0.24 |
ADDITIONAL EFFECT VALUES
- 16-h EC90: 3176.6 mg/L
Description of key information
The 16-h EC50 and EC10 values are 1628 and 769 mg/L as observed in a bacterial growth inhibition study (Pseudomonas putida).
Key value for chemical safety assessment
Additional information
The toxicity of the substance to microorganisms was investigated in a bacterial growth inhibition study according to guideline DIN 38412, part 8 (BASF, 1987). In this study, cultures of bacteria (Pseudomonas putida) were exposed to nominal test substance concentrations of 0 (control), 39.531, 78.125, 156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/L (based on a range-finding study) for 16 hours. Test concentrations were not analytically verified. Bacterial growth (inhibition) was determined optically by means of spectrophotometry at a wavelength of 436 nm and compared to a blank control. Up to and including the dose group of 625 mg/L no inhibition was observed. At a test concentration of 1250 mg/L, 34.19% cell growth inhibition was observed. At higher test concentrations the growth inhibition came close to 100%. Based on these findings, the 16-h EC50 and 16-h EC10 values were determined at 1628 mg/L and 769 mg/L, respectively.
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