N,N-diethyl-2-propynylamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 Sep 1989 to 27 Sep 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: DIN 38412 L.11

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in studyreport): Golpanol DEP
- Batch No.: 1/89

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTIONS
Test concentrations were prepared as dilutions from a 2000 mg/L stock solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Supplier: Bundesgesundheitsamt, Berlln
- Breeder: Dr.U.Noack-Laboratorium, Hildesheim

CULTURING
- Feed: Fresh algal mass (Scenedesmus subspicatus) was given once a day.
- Rearing: Single breed in 100 mL beaker with 60 mL breeding water.
- Water change: Water was changed three times per week (monday, wedenesday and friday).
- Temperature: 21 °C
- Ca/Mg ratio: 5:1
- Na/K ratio: 20:1
- Conductivity: 658 µS/cm
- Reproduction rate: 8 animals per day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21.0 °C

pH:

7.58 - 9.03. See 'Any other information on materials and methods incl. tables'

Dissolved oxygen:

8.4 - 8.5 mg O2/L

Conductivity:

658 µS/cm

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 32, 58, 100, 180, 320, 580 and 580 (neutralised pH) mg/L (based on a range-finding study).
- Measured concentrations: not determined.

Details on test conditions:

TEST SYSTEM
- Test vessel: Tall beakers with a nominal volume of 50 mL, containing 20 mL of a given test item concentration (DIN 12 331)
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to DIN 38412 part 11, 8.2
- Culture medium different from test medium: No
- Oxygen measurement: oxygen was measured using Oximeter, Oxi 191, Fa. WTW
- pH measurement: pH was measured using Oximeter, Oxi 191, Fa. WTW
- Temperature measurement: Temperature was measured using Oxi 191
- Conductivity: 658 µS/cm
- Ca/mg ratio: 5:1
- Na/K ratio: 20:1

OTHER TEST CONDITIONS
- Light intensity: 10 µE/qm X S

EFFECT PARAMETERS MEASURED: Immobility was determined after 3, 6, 24 and 48 hours exposure.

RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10, 50, 100 and 500 mg/L.
- Results used to determine the conditions for the definitive study: At study termination after 48 hours, all test animals in the group exposed to the highest concentration died (10/10). In the 100 mg/L group only one animal died after 48 hours exposure. No mortality was observed in the lower concentrations.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

129 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% C.I.: 109 - 152 mg/L

Details on results:

During exposure, no immobilisation was observed in the control. In the 32, 58, 100, 180, 320, 580 and 580 (neutralised pH) mg/L test groups, the immobilisation was 5, 10, 30, 75 and 100, 100 and 80%, respectively. A dose-dependent increase in immobility was observed with increasing test substance concentrations. An overview of the results is provided in 'Any other information on results incl. tables'.

Results with reference substance (positive control):

The test with the reference material potassium dichromate was performed from 26 Sep, 1989 to 27 Sep, 1989. The 24-h EC50 of the reference substance was determiend to be 0.9 mg/L.

Reported statistics and error estimates:

Spearman-Kärber method was used.

Table: Immobility observed throughout the study

Immobility [%]
Test concentration [mg/L]	3h					6 h					24 h					48 h
	R1	R2	R3	R4	mean	R1	R2	R3	R4	mean	R1	R2	R3	R4	mean	R1	R2	R3	R4	mean
580	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100
320	0	0	0	0	0	0	0	0	20	5	20	20	20	60	35	100	100	100	100	100
180	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	100	80	80	40	75
100	0	0	0	0	0	0	0	20	20	10	0	0	20	20	10	0	40	40	40	30
58	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	20	20	0	0	10
32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	20	0	0	0	5
Control	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
580 (pH neutralised)	0	0	0	0	0	0	0	0	0	0	0	20	0	0	5	100	60	100	60	80

ADDITIONAL EFFECT VALUES

3-h EC50: 450 mg/L (95% C.I.: 320 - 580 mg/L)

6-h EC50: 400 mg/L (95% C.I.: 320 - 580 mg/L)

24-h EC50: 352 mg/L (95% C.I.: 310 - 399 mg/L)

3-h EC0: 320 mg/L

6-h EC0: 320 mg/L

24-h EC0: 180 mg/L

48-h EC0: 58 mg/L

3-h EC100: 580 mg/L

6-h EC100: 580 mg/L

24-h EC100: 580 mg/L

48-h EC100: 320 mg/L

Validity criteria fulfilled:

yes

Remarks:

See 'Any other information on materials and methods incl. tables'

Description of key information

The 48-h EC50 value is 129 mg/L in aquatic invertebrates (Daphnia magna).

Additional information

The acute toxicity to aquatic invertebrates was determined in a study according to DIN 38412 L.11 (GLP not specified) (Dr. Noack Laboratorium, 1989). In this study, daphnids (D. magna, 20 per concentration) were exposed to nominal concentrations of 0 (control), 32, 58, 100, 180, 320, 580 and 580 (neutralised pH) mg/L for 48 hours under static conditions. As no analytical confirmation of the test concentrations was performed, the effect values were are expressed as nominal. Immobilization was recorded after 3, 6, 24 and 48 hours exposure. Throughout the study and at study termination, no immobilization was observed in the control treatment. Immobility recorded after 48 hours exposure increased with increasing test substance concentration from 5% in the lowest concentration treatment (32 mg/L) up to 100% immobilization in the highest concentration treatment (580 mg/L). Based on these findings, the 48-h EC50 value was determined at 129 mg/L.