N,N-diethyl-2-propynylamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 Feb 1988 to 4 Mar 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38412 L.1 and L.15

Version / remarks:

June, 1982

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Golpanol DEP
- Batch No.: 85-0337
- Degree of purity: > 98%
- Homogeneity: Ensured since the test substance is a genuine liquid
- Water solubility: 100 g/L

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance was added to the test water without any pretreatment.

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Consignment date: 10 Nov 1987
- Breeder and supplier: Fischzucht Paul Eggers
- Length at study initiation: 8.1 cm (range: 7.2 - 9.3 cm)
- Weight at study initiation: 5.3 g (range: 3.6 - 8.3 g)
- Copulence factor* of batch: 1.0 (determined at 25 Feb 1988)
* The corpulence factor (K) is calculated from the weight w (g) and the length l (cm) measured from the tip of the mouth to the end of the tail fin according to the formula K=100*w/l*3.

ACCLIMATION
The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Acclimation period: About 3 months
- Photoperiod: 16 hours light and 8 hours dark
- Total hardness: About 2.5 mmol/L
- Acid capacity: About 5.5 mmol/L
- Oxygen content: > 60% of maximum saturation
- pH: About 8.0
- Water temperature: 15 - 20 °C
- Mortality during the last 2 weeks of housing: 0%
- Mortality during the acclimation period: 0%
- Medical treatment: Twice with 0.05 mg/L malachite green chloride and once with 10 mg/L tetracycline hydrochloride
- Diet: Ad libitum growing feed F/B 50 (SSNIFF Spezialdiaeten GmbH, D-4770, Soest, FRG,)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/L

Test temperature:

20 - 21 °C

pH:

7.2 - 8.7

Dissolved oxygen:

7.4 - 9.3 mg/L

Salinity:

Resalting was carried out by the addition of the following:
- 194 mg/L CaCl2.2H2O
- 123.3 mg/L MgSO4.7H2O
- 64.8 mg/L NaHCO3
- 5.8 mg/L KCl

Conductivity:

10 µMho

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 10.0, 21.5, 46.4 and 100 mg/L (based on a range-finding study).
- Measured concentrations: not determined.

Details on test conditions:

TEST SYSTEM
- Test vessel: All-glass aquariums (30 cm x 22 cm x 24 cm) containing 10L test water.
- Aeration: slight.
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 5.3
- Adaptation to test water and test temperature: 3 days
- Withdrawal of food before test: 1 day

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38412, Part 11, Draft Sept. 1981. Preparation was done from fully demineralised tap water.
- Acid capacity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Intervals of water quality measurement: pH, oxygen content and temperature were measured after 1, 24, 48, 72 and 96 hours exposure.

EFFECT PARAMETERS MEASURED: Mortality and symptoms were determined 1, 4, 24, 48, 72 and 96 hours of exposure.

RANGE FINDING STUDY
- Results used to determine the conditions for the definitive study: The LC50 in the range finding study was determined to be between 10 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 46 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

67.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Geometric mean of LC0 and LC100 - determined by the assessor.

Details on results:

No mortality or symptoms were observed throughout the test except for the highest dose group of 100 mg/L. In the highest test group, all fish had died after 1 hour exposure (mortality was 100%). The NOEC is therefore 46.4 mg/L and the minimum concentration causing 100% mortality is 100 mg/L.

Results with reference substance (positive control):

The 48-h LC50 of the reference substance was determined to be about 32 mg/L (determined on 25 Jan 1988). This lethal concentration corresponds to normal sensitivity.

Reported statistics and error estimates:

If possible, the LC5 and LC95 were determined by the probit analysis after 1, 4, 24, 48, 72 and 96 hours.

Validity criteria fulfilled:

not specified

Description of key information

The 96-h LC50 value is > 46.0 (LC0) and < 100 mg/L (LC0) in fish (Leuciscus idus L.). The geometric mean of the LC0 and LC100 is 67.8 mg/L.

Additional information

The short-term toxicity to freshwater fish was determined in a study that closely followed DIN 38412 L.1 and L.15 (GLP not specified) (BASF, 1988). In this study, groups of 10 golden orfe (Leuciscus idus L.) were exposed to nominal test concentrations of 0 (control), 10.0, 21.5, 46.4 and 100 mg/L for 96 hours in static conditions. The test concentrations were selected based on the results of a range-finding study. Test concentrations were not analytically verified; therefore the effect values were expressed as nominal. No mortality and symptoms were observed in the control and all test groups, except for the test group exposed ot the highest concentration of 100 mg/L. At this concentration, mortality was 100% (10/10 fish died) after 1 hour exposure. Based on these findings, the 96-h LC50 is determined to be > 46 mg/L and < 100 mg/L. The geometric mean of the LC0 (46 mg/L) and LC100 (100 mg/L) is 67.8 mg/L (determined by the assessor).