Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 6341 15 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Version / remarks:
Method 6.1: 1996/Cor.1:1998
Deviations:
not specified
GLP compliance:
not specified
Remarks:
likely to be GLP but not specified in data available

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified
Details on test solutions:
No information is provided on how test solutions were prepared.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
No details available on source and use of Daphnia magna. Assumed neonates used were less than 24 hours old in accordance with the followed guidance.

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.7°C
pH:
6.0
Dissolved oxygen:
12.7 mg/L
Salinity:
< 1 ppt at 20°C
Conductivity:
560 µS/cm at 25°C
Nominal and measured concentrations:
Nominal Concentrations of 100, 180 and 320 mg/L are detailed.
Details on test conditions:
No detailed information on experimental conditions available.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
138 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Immobilisation results:

100 mg/L: No Daphnia immobilised.
180 mg/L: 95% of Daphnia immobilised.
320 mg/L: 100% of Daphnia immobilised.
Results with reference substance (positive control):
Not specified
Reported statistics and error estimates:
95% confidence limits of 130.5 to 146.2 (% vol/vol) calculated using Trimmed Spearman-Karber.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Under the conditions of the test conducted in accordance with ISO guidance provides a 48 hr EC50 value of 138 mg/L to Daphnia magna.