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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of fish for the test chemical :
1.To evaluate aquatic toxicity of test material on Daphnia magna
2.48 h immobilization bioassay was performed to evaluate potential of test material against Daphnia magna
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of the test chemical: Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate
- Molecular formula: C30H27ClN8O17S5.4Na
- Molecular weight: 1055.29 g/mol
- Substance type: Organic
- Smiles: [Na+].[Na+].[Na+].[Na+].c1c(c(c(c2c(cc(cc12)S(=O)(=O)[O-])NC(CC)=O)O)\N=N\c1c(ccc(c1)Nc1nc(nc(n1)Cl)Nc1cc(ccc1)S(=O)(=O)CCOS(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-]
- Inchi: 1S/C30H27ClN8O17S5.4Na/c1-2-24(40)34-21-14-19(58(44,45)46)10-15-11-23(60(50,51)52)26(27(41)25(15)21)39-38-20-13-17(6-7-22(20)59(47,48)49)33-30-36-28(31)35-29(37-30)32-16-4-3-5-18(12-16)57(42,43)9-8-56-61(53,54)55;;;;/h3-7,10-14,41H,2,8-9H2,1H3,(H,34,40)(H,44,45,46)(H,47,48,49)(H,50,51,52)(H,53,54,55)(H2,32,33,35,36,37);;;;/q;4*+1/p-4/b39-38+;;;;
Analytical monitoring:
not specified
Details on sampling:
No data available
Vehicle:
not specified
Details on test solutions:
1.- Sample storage conditions before analysis: effluent samples were stored in amber glass bottles(2.0 L), transported to the laboratory well before 48-h in a lightproof ice chest, stored under refrigeration (at 4 °C) in darkness until analysis
Test organisms (species):
Daphnia magna
Details on test organisms:
1.- Common name: Daphnia magna
- Strain: Daphnia magna
- Source: laboratory stock culture
- Age at study initiation: 24 hr old neonatal
- Feeding during test : 24-h before experiment
- Food type: Selenastrum sp. and yeast powder

2.- Common name:
- Strain: Daphnia magna
- Source: Daphtoxkit F™ magna, a commercial kit that provides daphnids in the form of dormant eggs (ephippia)
- Age at study initiation : 24 h old neonates
- Feeding during test: prior 2 days of test
- Food type: lyophilized spirulina

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
1)25 ± 2.0 °C
2) 20±1 °C
pH:
1) 7.8
Nominal and measured concentrations:
1) 100, 80, 60, 40, 20, and 10 mg/L
2) 100mg/l
Details on test conditions:
1)- Test vessel: 15 mL glass test tube
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
3.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Read across 1
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.271 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: confidence interval (C.I.)-3.680-4.957
Remarks:
Read across 2
Results with reference substance (positive control):
potassium dichromate : 0.6-2.1 mg/l
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2- (sulphonatooxy)ethyl]sulphonyl]phenyl]amino]- 1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5 -[(1-oxopropyl)amino]naphthalene-2,7-disulphonate is likely to be toxic to Daphnia magna in the concentration range of 3.6-4.27 mg/l
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2- (sulphonatooxy)ethyl]sulphonyl]phenyl]amino]- 1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5 -[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment.

Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L

The LC 50 value for the test chemical was observed to be 24-h LC50 6.0 mg/L;48-h LC50 3.6–6.0 mg/L.

2.The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test. The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957 .

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and itsstructurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 3.6-4.27   mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 3.6-4.27   mg/L .

Description of key information

Short-term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2- (sulphonatooxy)ethyl]sulphonyl]phenyl]amino]- 1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5 -[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.Test material when used as a test material to evaluate its aquatic toxicity on Da4phnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment.

Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L

The LC 50 value for the test chemical was observed to be 24-h LC50 6.0 mg/L;48-h LC50 3.6–6.0 mg/L.

2.The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test.The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957.

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and itsstructurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 3.6-4.27   mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 3.6-4.27   mg/L .

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
4.271 mg/L

Additional information

Short-term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2- (sulphonatooxy)ethyl]sulphonyl]phenyl]amino]- 1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5 -[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment.

Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L

The LC 50 value for the test chemical was observed to be 24-h LC50 6.0 mg/L;48-h LC50 3.6–6.0 mg/L.

2.The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test.The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957.

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and itsstructurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 3.6-4.27   mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 3.6-4.27   mg/L .