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Environmental fate & pathways

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Biodegradation in water:

Estimation Programs Interface Suite (EPI suite, 2018) was run to predict the biodegradation potential of the test compound Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7 -disulphonate

(CAS no. 80019 -42 -7) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-

2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate is expected to be not readily biodegradable.

Additional information

Biodegradation in water:

Predicted data study for target chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7 -disulphonate (CAS no. 80019 -42 -7) and experimental studies for its structurally similar red across chemical have been reviewed for biodegradation endpoint and their results are summarized below.

 

First study was predicted data study in this study the Estimation Programs Interface Suite (EPI suite, 2018) was run to predict the biodegradation potential of the test compound Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7 -disulphonate (CAS no. 80019 -42 -7) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]- 2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate is expected to be not readily biodegradable.

 

Next study was experimental study reviewed from journal in this study the Biodegradation experiment was carried out for 42 days for evaluating the percentage biodegradation of the test chemical using modified OECD Guideline 302B. Activated sludge was used as a test inoculum.The sources of the activated sludge were treatment plants conveniently located to the laboratories carrying out the test. These treatment plants received communal and/or industrial wastewater. Concentration of inoculum i.e, activated sludge used was 0.5 g/l and initial test substance conc. used in the study was 100 mg/l. Analytical methods involve the measurement of extinction at absorption maximum 412 nm and DOC (dissolved organic carbon). The percentage degradation of the test substance was determined to be -17% by using DOC removal parameter in 42 days. Thus, based on percentage degradation, the test chemical was considered to be not readily biodegradable in nature.

 

Further to support predicted data study of target chemical experimental study was reviewed from authoritative databases in this study the Biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)) under aerobic conditions. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 4, 1 and 2 % by BOD, TOC removal and HPLC parameter respectively in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

Last study was also experimental study reviewed from journal the aim of the study was to estimate the microbial decomposition of test chemical by sludge under aerobic conditions.

Return activated sludge was obtained from the municipal sewage treatment plant, Nakahama, Osaka.

Synthetic sewage preparation: Glucose , peptone and potassium dihydrogen phosphate, 30g each, were dissolved in 1 liter water and the pH was adjusted to pH 7.0 with sodium hydroxide

Seeded Dilution water: To 1 liter, 10 ml of supernatant of sludge was added.

Aerobic biodegradation assay: To 750ml of sludge (MLSS ca, 3,000 ppm) 250 ml of O.03 M dye solution was added, and bubbled with air sufficiently at 20°C. 5ml sample was taken out once a day. After sampling done 5ml of synthetic sewage was added to the mixture. Each sample was filtered through filter paper and diluted twenty times prior to the spectrophotometric measurement at the absorption maximum within the visible range. The decrease of dyes concentration was expressed in terms of percent to the initial absorption. The experiment was carried out for 10 days.

Oxygen uptake of sludge [Warburg Method]- 2.0 ml of sludge, 0.2 ml of 1000 ppm dye solution, and 0.2 ml of 20% potassium hydroxide were pipetted into the vessel, the side arm and central well, respectively. The sludge and the dye solution were mixed and the vessel was shaken at 25°C. The oxygen uptake was measured.

The oxygen uptake by sludge alone was subtracted from those by dyes addition.

During 10 days aerobic experiment, test chemical was decomposed about 20 % in 10 days

From the oxygen uptake by Warburg’s manometer, the low activity of the sludge to dye was obtained.

Based on the results obtained from the aerobic degradation assay and Warburg method, it can be concluded that test chemical is not readily biodegradable under aerobic conditions.

 

By considering results of all the studies mentioned above for target chemical and its read across chemical it can be concluded that test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7 -disulphonate (CAS no. 80019 -42 -7) is expected to be not readily biodegradable in nature.