1,1'-[(3-{bis[3-(dimethylamino)propyl]amino}propyl)imino]dipropan-2-ol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Four samples of 6 mL were taken from the additional vessels (without test organisms in order to not disturb the TOC-analysis) of treatment A and NC after 0 h, 48 h and 72 h exposure: 4 samples per treatment and time.
Of the 4 samples per treatment and time, 2 were analysed directly after sampling using a TOCanalyser (Non Purgeable Organic Carbon [NPOC] method) and 2 were stored as retain samples in the freezer (≤ -18 °C) until finalisation of the study.

Details on test solutions:

The stock solutions were prepared by adding 80 mg test item to 8 L test medium and stirring for 0.5 h using a magnetic stirrer. This stock solution is used as single test item concentration in the test according to the following scheme. One replicate with seven fish was prepared for the test item treatment as well as the negative control (NC).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The test organism Danio rerio originates from the in-house breeding of Hydrotox GmbH. The zebrafish are cultured at 26 ± 2 °C with 14 h light : 10 h dark and aeration with air pumps. They are kept in glass tanks, equipped with external filters, at ≤ 1 fish/L. On workdays, they are fed with commercial fish food (TetraMin flake food, Tetra GmbH, Melle, Germany) and two times per week with freshly hatched Artemia salina larvae. At least every two weeks, ≥ ⅓ of the tank volume is
replaced with fresh charcoal filtered tap water. The used test animals had a total length of ca. 2.5 – 3 cm and were ca. 10 months old.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

The temperature during the exposure was 22.8 – 23.7 °C in the control and 22.8 – 23.7 °C in the test item treatment (required 21 – 25 °C).

pH:

The pH was 7.6 – 7.8 in the control and 7.7 – 8.1 in the test item treatment.

Dissolved oxygen:

The dissolved oxygen concentration was 8.1 – 8.2 mg/L in the control and 8.2 – 8.3 mg/L in the test item treatment (> 60 % of the air saturation).

Conductivity:

The conductivity was 702 – 728 μS/cm in the control and 694 – 706 μS/cm in the test item treatment.

Details on test conditions:

Seven days before the start of the test, the fish were kept under test conditions in test medium at 22.8 – 24.7 °C. They were fed every working day with commercial fish food (TetraMin) until 24 h before test start, when feeding was stopped.
The test vessels were set up in a climatised room with 14 h light : 10 h dark. A static test design was applied. The pH, conductivity and O2-concentrations were measured daily. The temperature was measured continuously.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Key result

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

Mortality
As no fish died until the end of the exposure, the mortality in the control as well as in the test item treatment was 0 %.

Appearance and behaviour
No obvious effect on appearance and behaviour of the fish in the test item treatment was observable. All test organisms in the control as well as in the test item treatment appeared healthy and lively throughout the exposure.

Effect concentrations
As no effect on the test organisms was observable, no statistical data evaluation was performed.

Validity criteria fulfilled:

yes

Conclusions:

As the measured test item concentrations are within ± 20 % of the nominal concentration, according to OECD 203 (1992), all results are given in relation to the nominal test item concentration.
LOEC > 10 mg/L
NOEC ≥ 10 mg/L

Executive summary:

LOEC > 10 mg/L

NOEC ≥ 10 mg/L

Description of key information

As the measured test item concentrations are within ± 20 % of the nominal concentration, according to OECD 203 (1992), all results are given in relation to the nominal test item concentration.

LOEC > 10 mg/L

NOEC ≥ 10 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10 mg/L