Registration Dossier

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
not applicable
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3,4-Thiadiazole-2(3H)-thione, 5-(tert-dodecyldithio)-
EC Number:
813-543-0
Cas Number:
73984-93-7
Molecular formula:
C14H26N2S4
IUPAC Name:
1,3,4-Thiadiazole-2(3H)-thione, 5-(tert-dodecyldithio)-
Test material form:
liquid

Test animals

Species:
rat
Strain:
Crj: CD(SD)
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Charles River
- Age at study initiation: not reported
- Weight at study initiation: 264 to 284g (males) and 169 to 182g (females)
- Housing: individually in wire-mesh cages
- Diet (e.g. ad libitum): Purina laboratory chow was available ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): during the 4 h exposure period the temperature in the exposure chamber was 27°C
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported

IN-LIFE DATES:
From: 15 Dec 1978 To: 29 Dec 1978

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
The vapours of the compound were generated by passing air at the rate of 10 L/min through a round bottom flask containing the compound. The flask was placed in a heating jacket heated to 93°C. The vapours emerging from the flask were introduced into the exposure chamber without dilution. The temperature within the exposure chamber was approximately 27° C. The rats were thus exposed to an atmosphere essentially saturated with vapours of the test material. The concentration of the vapour (0.62 mg/L) in the chamber atmosphere was calculated from the ratio of the total weight loss of the material in the flask after the exposure (1.49 grams) to the total volume of air passing through the flask during the 4 hour period (2400 litres).

Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
One exposure level of 0.62 mg/L was used
No. of animals per sex per dose:
5 males and 5 females were used
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations were performed twice daily during the 14 day observation period and body weights were performed at Day 0 (prior to exposure), 1, 3, 5 ,7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 0.62 mg/L air (nominal)
Based on:
test mat.
Exp. duration:
4 h
Mortality:
No mortality occured during the study
Clinical signs:
other: The immediate response of the rats to the experimental atmosphere was an increase of activity in preening. After several minutes of exposure this activity decreased. The rats appeared normal during the 4 hour exposure and throughout the 14 day observati
Body weight:
No effect on body weight was noted during the study, refer to table below.
Gross pathology:
No findings noted

Any other information on results incl. tables

 

Animal No.

Body weight (g)

Day 0

Day 1

Day 3

Day 5

Day 7

Day 14

M9356

M9357

M9358

M9359

M9360

278

282

276

264

284

280

283

275

259

278

286

280

277

260

280

289

277

279

259

280

286

283

285

265

284

288

287

296

275

284

F9361

F9362

F9363

F9364

F9365

182

172

177

178

169

182

168

173

180

169

180

167

173

180

168

177

167

168

175

165

182

168

171

178

168

182

174

170

178

169

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Executive summary:

An acute inhalation study was conducted using a similar procedure to that described in the current OECD Guideline 403.

The test material (CAS No. 50530 -43 -3) was administered at 0.62 mg/L by inhalation for 4 h to five male and five female rats. The rats were observed for 14 days after test material administration. No mortalities occurred during the study. No gross pathologic alterations were noted among the animals.

The acute inhalation median lethal dose (LD50) was estimated to be greater than 0.62 mg/L.